Anti-IGFBP3抗体(ab77635)
Key features and details
- Goat polyclonal to IGFBP3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IGFBP3抗体
参阅全部 IGFBP3 一抗 -
描述
山羊多克隆抗体to IGFBP3 -
宿主
Goat -
特异性
ab77635 is expected to recognize both reported isoforms (NP_001013416.1 and NP_000589.2). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Cow, Dog
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免疫原
Synthetic peptide:
C-RYKVDYESQSTDTQN
, from the internal region of human IGFBP3 (NP_001013416.1; NP_000589.2). -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab77635 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77635于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
| 说明 |
|---|
应用说明
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
组织特异性
Expressed by most tissues. Present in plasma. -
序列相似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
发展阶段
IGFBP3 levels are higher during extrauterine life and peak during puberty. -
结构域
The thyroglobulin type-1 domain mediates interaction with HN. -
翻译后修饰
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3486 Human
- Entrez Gene: 16009 Mouse
- Entrez Gene: 24484 Rat
- Omim: 146732 Human
- SwissProt: P20959 Cow
- SwissProt: P17936 Human
- SwissProt: P47878 Mouse
- SwissProt: P15473 Rat
see all -
别名
- Acid stable subunit of the 140 K IGF complex antibody
- Binding protein 29 antibody
- Binding protein 53 antibody
see all
图片
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Western blot - Anti-IGFBP3 antibody (ab77635)All lanes : Anti-IGFBP3 antibody (ab77635) at 0.02 µg/ml
Lane 1 : human breast cancer lysate (in RIPA buffer)
Lane 2 : human breast cancer lysate (in RIPA buffer) with immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 32 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific binding)
The additional band of 50 kDa was consistently observed. However this band was not blocked by the immunizing peptide and it is therefore a non specific signal.
数据表及文件
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Datasheet download
文献 (7)
ab77635 被引用在 7 文献中.
- Thiebaut AM et al. Thrombolysis by PLAT/tPA increases serum free IGF1 leading to a decrease of deleterious autophagy following brain ischemia. Autophagy 18:1297-1317 (2022). PubMed: 34520334
- Nery FC et al. Impaired kidney structure and function in spinal muscular atrophy. Neurol Genet 5:e353 (2019). PubMed: 31517062
- Bai R et al. The NF-?B-modulated miR-19a-3p enhances malignancy of human ovarian cancer cells through inhibition of IGFBP-3 expression. Mol Carcinog 58:2254-2265 (2019). PubMed: 31513316
- Yao TH et al. Propranolol induces hemangioma endothelial cell apoptosis via a p53-BAX mediated pathway. Mol Med Rep 18:684-694 (2018). PubMed: 29767244
- Marthandan S et al. Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence. Biol Res 49:34 (2016). PubMed: 27464526
- Goyal R et al. Antenatal maternal long-term hypoxia: acclimatization responses with altered gene expression in ovine fetal carotid arteries. PLoS One 8:e82200 (2013). WB ; Sheep . PubMed: 24367503
- Yoo EG et al. Insulin-like growth factor-binding protein-3 mediates high glucose-induced apoptosis by increasing oxidative stress in proximal tubular epithelial cells. Endocrinology 152:3135-42 (2011). WB ; Pig . PubMed: 21652730
