重组Anti-IGFBP2抗体[EPR3380(2)] (ab109284)
![Western blot - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)](https://www.abcam.cn/ps/products/109/ab109284/Images/ab109284-122017-anti-igfbp2-antibody-epr33802-western-blot.jpg "Western blot - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3380(2)] to IGFBP2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IGFBP2抗体[EPR3380(2)]
参阅全部 IGFBP2 一抗 -
描述
兔单克隆抗体[EPR3380(2)] to IGFBP2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human kidney tissue, Human endometrial adenocarcinoma tissue. T47-D, SH-SY5Y, BxPC-3, A549 and NCCIT cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3380(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109284于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 35 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.
靶标
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功能
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
序列相似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
结构域
The C-terminus is required for IGF-binding and growth inhibition. -
翻译后修饰
O-glycosylated. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3485 Human
- Omim: 146731 Human
- SwissProt: P18065 Human
- Unigene: 438102 Human
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别名
- BP 2 antibody
- BP2 antibody
- IBP 2 antibody
see all
图片
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Western blot - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)All lanes : Anti-IGFBP2 antibody [EPR3380(2)] (ab109284) at 1/10000 dilution
Lane 1 : T47-D cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : NCCIT cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)](https://www.abcam.cn/ps/products/109/ab109284/Images/ab109284-122016-anti-igfbp2-antibody-epr33802-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)Immunohistochemical analysis of paraffin-embedded kidney tissue using 1/100 ab109284.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)](https://www.abcam.cn/ps/products/109/ab109284/Images/ab109284-122015-anti-igfbp2-antibody-epr33802-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)Immunohistochemical analysis of paraffin-embedded endometrial adenocarcinoma tissue with ab109284 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)](https://www.abcam.cn/ps/products/109/ab109284/Images/ab109284-4-benefits-of-recombinant-antibodies.png)
Anti-IGFBP2 antibody [EPR3380(2)] (ab109284)
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab109284 被引用在 8 文献中.
- Masroni MSB et al. Dynamic altruistic cooperation within breast tumors. Mol Cancer 22:206 (2023). PubMed: 38093346
- Sato Y et al. IGFBP1 Is a Predictive Factor for Haematogenous Metastasis in Patients With Gastric Cancer. Anticancer Res 39:2829-2837 (2019). PubMed: 31177120
- Gao C et al. Adeno-associated virus type 2-mediated gene transfer of a short hairpin-RNA targeting human IGFBP-2 suppresses the proliferation and invasion of MDA-MB-468 cells. Mol Med Rep 17:4383-4391 (2018). PubMed: 29344663
- Strub T et al. SIRT6 haploinsufficiency induces BRAFV600E melanoma cell resistance to MAPK inhibitors via IGF signalling. Nat Commun 9:3440 (2018). PubMed: 30143629
- Zhao S et al. Downregulation of CD147 induces malignant melanoma cell apoptosis via the regulation of IGFBP2 expression. Int J Oncol 53:2397-2408 (2018). PubMed: 30272281
- Huang YF et al. Vitamin D-Binding Protein Enhances Epithelial Ovarian Cancer Progression by Regulating the Insulin-like Growth Factor-1/Akt Pathway and Vitamin D Receptor Transcription. Clin Cancer Res 24:3217-3228 (2018). PubMed: 29476020
- Nawathe AR et al. Insulin-like growth factor axis in pregnancies affected by fetal growth disorders. Clin Epigenetics 8:11 (2016). WB ; Human . PubMed: 26823688
- Wang C et al. Human adipocytes stimulate invasion of breast cancer MCF-7 cells by secreting IGFBP-2. PLoS One 10:e0119348 (2015). WB ; Human . PubMed: 25747684