重组Anti-IGF2BP1 + IGF2BP2抗体[EPR6740(2)] (ab129071)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6740(2)] to IGF2BP2/IMP-2 + IGF2BP1/IMP1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IGF2BP1 + IGF2BP2抗体[EPR6740(2)] -
描述
兔单克隆抗体[EPR6740(2)] to IGF2BP2/IMP-2 + IGF2BP1/IMP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human IGF2BP2/IMP-2 aa 100-200. The exact sequence is proprietary.
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阳性对照
- 293T and Caco 2 cell lysates; Human colon tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.00 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6740(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129071于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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细胞定位
IGF2BP2/IMP-2: Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. IGF2BP1/IMP1: Nucleus. Cytoplasm. Cell projection > lamellipodium. Cell projection > dendrite. Cell projection > dendritic spine. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Targeted to stress granules (SGs), but not processing bodies (PBs), during cellular stress. Colocalizes with G3BP1 and TIAL1 in SGs. Colocalizes with HIV-1 GAG at the cell edges. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Colocalized with H19 RNA at lamellipodia. Colocalized with CD44 mRNA in RNP granules. Nuclear export is mediated by XPO1/CRM1. In motile cells, is transported towards the leading edge into the cortical region of the lamellipodia where it is connected to microfilaments (By similarity). Present in the form of granules and into F-actin-rich protrusion of dendrites, spines and subsynaptic sites (By similarity). Colocalizes with beta-actin mRNA in dendrites and spines (By similarity). Exhibited rapid, bidirectional movements in dendrites and spines (By similarity). Neuronal depolarization by KCl induces its rapid efflux from the cell body into dendrites. -
数据库链接
- Entrez Gene: 10642 Human
- Entrez Gene: 10644 Human
- Entrez Gene: 140486 Mouse
- Entrez Gene: 319765 Mouse
- Entrez Gene: 303477 Rat
- Entrez Gene: 303824 Rat
- Omim: 608288 Human
- Omim: 608289 Human
see all
图片
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All lanes : Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071) at 1/1000 dilution
Lane 1 : C-Myc/DDK -tagged full length Human IGF2BP1 recombinant protein, 10ng
Lane 2 : C-Myc/DDK -tagged full length Human IGF2BP2 recombinant protein, 10ng
Lane 3 : N-His -tagged full length Human IGF2BP3 recombinant protein, 10ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 66 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1 + IGF2BP2 antibody [EPR6740(2)] (ab129071)
ab129071, at 1/50 dilution, staining IGF2BP1 + IGF2BP2 in paraffin-embedded Human colon tissue by Immunohistochemistry
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab129071 被引用在 7 文献中.
- Zhang T et al. IGF2BP2 enhances LincRNA01116 stability via m6 A: A potential biomarker and therapeutic target for patients with pre-eclampsia. J Cell Biochem 124:239-253 (2023). PubMed: 36538649
- Tang H et al. Comprehensive analysis of the expression of the IGF2BPs gene family in head and neck squamous cell carcinoma: Association with prognostic value and tumor immunity. Heliyon 9:e20659 (2023). PubMed: 37842569
- Ou M et al. HOXA cluster antisense RNA 2 elevates KIAA1522 expression through microRNA-520d-3p and insulin like growth factor 2 mRNA binding protein 3 to promote the growth of vascular smooth muscle cells in thoracic aortic aneurysm. ESC Heart Fail 9:2955-2966 (2022). PubMed: 35730141
- Zhi S et al. Insulin-like growth factor 2 mRNA binding protein 2 regulates proliferation, migration, and angiogenesis of keratinocytes by modulating heparanase stability. Bioengineered 12:11267-11276 (2021). PubMed: 34753397
- Wang S et al. RNA-binding protein IGF2BP2 enhances circ_0000745 abundancy and promotes aggressiveness and stemness of ovarian cancer cells via the microRNA-3187-3p/ERBB4/PI3K/AKT axis. J Ovarian Res 14:154 (2021). PubMed: 34774079
- Yuan X et al. Human umbilical cord mesenchymal stem cells deliver exogenous miR-26a-5p via exosomes to inhibit nucleus pulposus cell pyroptosis through METTL14/NLRP3. Mol Med 27:91 (2021). PubMed: 34412584
- McMullen ER et al. CCN6 regulates IGF2BP2 and HMGA2 signaling in metaplastic carcinomas of the breast. Breast Cancer Res Treat 172:577-586 (2018). PubMed: 30220054