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重组RabMAb

重组Anti-IGF2抗体[EPR25955-88] - BSA and Azide free (ab317491)

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Western blot - Anti-IGF2 antibody [EPR25955-88] - BSA and Azide free (ab317491)
  • Western blot - Anti-IGF2 antibody [EPR25955-88] - BSA and Azide free (ab317491)
  • Immunoprecipitation - Anti-IGF2 antibody [EPR25955-88] - BSA and Azide free (ab317491)
  • Anti-IGF2 antibody [EPR25955-88] - BSA and Azide free (ab317491)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25955-88] to IGF2 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-IGF2抗体[EPR25955-88] - BSA and Azide free
    参阅全部 IGF2 一抗

  • 描述

    兔单克隆抗体[EPR25955-88] to IGF2 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IP, WBmore details
    不适用于: Flow Cyt (Intra),ICC/IF or IHC-P

  • 种属反应性

    与反应: Mouse
    不与反应: Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse placenta and Mouse IGF2 active protein. IP: Mouse placenta tissue lysate

  • 常规说明

    ab317491 is the carrier-free version of ab317490.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317491于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
说明
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt (Intra),ICC/IF or IHC-P.

靶标

  • 功能

    The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.

  • 疾病相关

    Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.

  • 序列相似性

    Belongs to the insulin family.

  • 翻译后修饰

    O-glycosylated with a core 1 or possibly core 8 glycan.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • C11orf43 antibody
      • IGF 2 antibody
      • IGF II antibody
      • IGF-II antibody
      • IGF2 antibody
      • IGF2_HUMAN antibody
      • IGFII antibody
      • INSIGF antibody
      • insulin like growth factor 2 (somatomedin A) antibody
      • Insulin like Growth Factor 2 antibody
      • Insulin like growth factor II antibody
      • Insulin like growth factor II precursor antibody
      • Insulin like growth factor type 2 antibody
      • pp9974 antibody
      • Preptin antibody
      • putative insulin like growth factor II associated protein antibody
      • Somatomedin A antibody
      • Somatomedin-A antibody
      see all

    图片

    • Western blot - Anti-IGF2 antibody [EPR25955-88] - BSA and Azide free (ab317491)
      Western blot - Anti-IGF2 antibody [EPR25955-88] - BSA and Azide free (ab317491)
      All lanes : Anti-IGF2 antibody [EPR25955-88] (ab317490) at 1/1000 dilution

      Lane 1 : Mouse placenta tissue lysate at 30 µg
      Lane 2 : Mouse pancreas tissue lysate at 20 µg
      Lane 3 : Mouse kidney tissue lysate at 20 µg
      Lane 4 : Mouse liver tissue lysate at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 18 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab317490, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: pancreas, kidney, liver.

      The identity of the bands higher than 25 kDa are unknown.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-IGF2 antibody [EPR25955-88] - BSA and Azide free (ab317491)
      Western blot - Anti-IGF2 antibody [EPR25955-88] - BSA and Azide free (ab317491)
      All lanes : Anti-IGF2 antibody [EPR25955-88] (ab317490) at 1/1000 dilution

      Lane 1 : Mouse IGF2 active protein 10 ng
      Lane 2 : Mouse IGF1 active protein 10 ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 8 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      This data was developed using ab317490, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      This antibody does not cross-react with mouse IGF1.

      In Western blot, Anti-IGF1 + IGF2 antibody -  staining at 1/500 dilution.

    • Immunoprecipitation - Anti-IGF2 antibody [EPR25955-88] - BSA and Azide free (ab317491)
      Immunoprecipitation - Anti-IGF2 antibody [EPR25955-88] - BSA and Azide free (ab317491)

      This data was developed using ab317490, the same antibody clone in a different buffer formulation.

      IGF2 was immunoprecipitated from 0.35 mg Mouse placenta tissue lysate with ab317490 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317490 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse placenta tissue lysate
      Lane 2: ab317490 IP in Mouse placenta tissue lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317490 in mouse placenta tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 24 seconds..

    • Anti-IGF2 antibody [EPR25955-88] - BSA and Azide free (ab317491)
      Anti-IGF2 antibody [EPR25955-88] - BSA and Azide free (ab317491)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317491？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317491 尚未被引用在任何文献中。

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