Anti-IGF2抗体(ab9574)
Key features and details
- Rabbit polyclonal to IGF2
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IGF2抗体
参阅全部 IGF2 一抗 -
描述
兔多克隆抗体to IGF2 -
宿主
Rabbit -
经测试应用
适用于: WB, Sandwich ELISAmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human IGF2. Highly pure (>98%) recombinant hIGF-2 (human Insulin Like Growth Factor-2).
Database link: P01344 -
常规说明
Although some customers have been successful in IHC we no longer batch test in this application.
Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C -
存放说明
Shipped at 4°C. Store at -20ºC. -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (6) |
Use at an assay dependent concentration. To detect hIGF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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| Sandwich ELISA |
Use at an assay dependent concentration.
To detect hIGF-II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83142, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II. |
| 说明 |
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WB
Use at an assay dependent concentration. To detect hIGF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Sandwich ELISA
Use at an assay dependent concentration. To detect hIGF-II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83142, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II. |
靶标
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功能
The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. -
疾病相关
Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. -
序列相似性
Belongs to the insulin family. -
翻译后修饰
O-glycosylated with a core 1 or possibly core 8 glycan. -
细胞定位
Secreted. - Information by UniProt
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别名
- C11orf43 antibody
- IGF 2 antibody
- IGF II antibody
see all
图片
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Sandwich ELISA - Anti-IGF2 antibody (ab9574)
ab9574 detecting IGF2 using sandwich ELISA
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Western blot - Anti-IGF2 antibody (ab9574)Rabbit Anti-Human IGF-II against Human IGF-II. Antibody concentration: 0.10 ug/ml Lower limit of detection: 3.91 ng/lane Molecular Weight: 7.5 kDa
Lane 1: Marker
Lane 2: 250 ng
Lane 3: 125 ng
Lane 4: 62.5 ng
Lane 5: 31.25 ng
Lane 6: 15.625 ng
Lane 7: 7.8 ng
Lane 8: 3.9 ng
实验方案
数据表及文件
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Datasheet download
文献 (108)
ab9574 被引用在 108 文献中.
- Liu X et al. Brain-derived extracellular vesicles promote bone-fat imbalance in Alzheimer's disease. Int J Biol Sci 19:2409-2427 (2023). PubMed: 37215980
- Zhou X et al. IGF2 deficiency promotes liver aging through mitochondrial dysfunction and upregulated CEBPB signaling in D-galactose-induced aging mice. Mol Med 29:161 (2023). PubMed: 38017373
- Tcw J et al. Cholesterol and matrisome pathways dysregulated in astrocytes and microglia. Cell 185:2213-2233.e25 (2022). PubMed: 35750033
- Sepúlveda D et al. Insulin-like growth factor 2 and autophagy gene expression alteration arise as potential biomarkers in Parkinson's disease. Sci Rep 12:2038 (2022). PubMed: 35132125
- Yu Y et al. Circ_0026579 alleviates LPS-induced WI-38 cells inflammation injury in infantile pneumonia. Innate Immun 28:37-48 (2022). PubMed: 35142578
