Anti-IGF1+IGF2抗体(ab9572)
Key features and details
- Rabbit polyclonal to IGF1 + IGF2
- Suitable for: ICC/IF, IHC-FoFr, WB, Neutralising, IHC-P, Sandwich ELISA
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IGF1+IGF2抗体 -
描述
兔多克隆抗体to IGF1 + IGF2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-FoFr, WB, Neutralising, IHC-P, Sandwich ELISAmore details -
种属反应性
与反应: Mouse, Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human IGF1. Highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1).
Database link: P05019 -
阳性对照
- IHC-P: Human pancreas and liver tissues.
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常规说明
Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see ab263903
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
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sELISA pair antibody
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
Use at an assay dependent concentration.
|
| IHC-FoFr | (1) |
Use at an assay dependent concentration. PubMed: 28754163
|
| WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required. |
|
| IHC-P | (1) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to IGF1 (ab83137). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1.
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-FoFr
Use at an assay dependent concentration. PubMed: 28754163 |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required. |
|
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to IGF1 (ab83137). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1. |
靶标
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细胞定位
IGF1: Secreted. IGF2: Secreted. -
数据库链接
- Entrez Gene: 3479 Human
- Entrez Gene: 3481 Human
- Entrez Gene: 16000 Mouse
- Entrez Gene: 16002 Mouse
- Omim: 147440 Human
- Omim: 147470 Human
- SwissProt: P01343 Human
- SwissProt: P01344 Human
see all -
形式
IGF1: There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
图片
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Western blot - Anti-IGF1+IGF2 antibody (ab9572)All lanes : Anti-IGF1+IGF2 antibody (ab9572) at 1/1000 dilution
Lanes 1 & 3 : Mouse IGF-2 recombinant protein (aa25-91, without tag)
Lanes 2 & 4 : Mouse IGF-1 recombinant protein (aa25-91, without tag)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Exposure time: 59 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1+IGF2 antibody (ab9572)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling IGF1 with ab9572 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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Sandwich ELISA - Anti-IGF1+IGF2 antibody (ab9572)To detect hIGF-I by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-I as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1+IGF2 antibody (ab9572)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IGF1 with ab9572 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
数据表及文件
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Datasheet download
文献 (114)
ab9572 被引用在 114 文献中.
- Wu C et al. Assessment of stromal SCD-induced drug resistance of PDAC using 3D-printed zPDX model chips. iScience 26:105723 (2023). PubMed: 36590169
- Zhou J et al. Microglial CD11b Knockout Contributes to Axonal Debris Clearance and Axonal Degradation Attenuation via IGF-1 After Acute Optic Nerve Injury. Invest Ophthalmol Vis Sci 64:7 (2023). PubMed: 37145604
- Chen M et al. EGFR marks a subpopulation of dermal mesenchymal cells highly expressing IGF1 which enhances hair follicle regeneration. J Cell Mol Med 27:1697-1707 (2023). PubMed: 37165726
- Wade H et al. Akkermansia muciniphila and its membrane protein ameliorates intestinal inflammatory stress and promotes epithelial wound healing via CREBH and miR-143/145. J Biomed Sci 30:38 (2023). PubMed: 37287024
- Fiore NT et al. Differential Effects of Regulatory T Cells in the Meninges and Spinal Cord of Male and Female Mice with Neuropathic Pain. Cells 12:N/A (2023). PubMed: 37759539
