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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases

Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163)抗体(ab5681)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (10)

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Western blot - Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody (ab5681)

    Key features and details

    • Rabbit polyclonal to IGF1 Receptor (phospho Y1131 + Y1135 + Y1136) + Insulin Receptor (phospho Y1158 + Y1162 + Y1163)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163)抗体
    • 描述

      兔多克隆抗体to IGF1 Receptor (phospho Y1131 + Y1135 + Y1136) + Insulin Receptor (phospho Y1158 + Y1162 + Y1163)
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat
    • 免疫原

      Synthetic peptide corresponding to IGF1 Receptor (phospho Y1158 + Y1162 + W1163).

    • 阳性对照

      • WB: CHO-T (Chinese hamster ovary cell line) whole cell extract transfected with a vector encoding the human insulin receptor and stimulated with insulin.
    • 常规说明

      The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1158, 1162 and 1163 of the human insulin receptor (IR) as numbered according to Ebina, et al. (1146, 1150 and 1151 according to Ullrich, et al.). The corresponding residues in the IGF1R are 1131, 1135 and 1136. The sequence is conserved in human, mouse and rat for both the IR and IGF1R.The two relevant papers are:Ebina, Y., et al. (1985) The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling. Cell 40(4):747-758.Ullrich, A., et al. (1985) Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes. Nature 313(6005):756-761. There are a long and a short isoform of this protein. This is why we are listing 1158, 1162 and 1163 in the name (where these phospho sites in the long isoform) as well as 1146, 1150 and 1151 (for the short isoform).

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Insulin Receptor (IR). The final product is generated by affinity chromatography using an IR-derived peptide phosphorylated at tyrosines 1158, 1162 and 1163 (1131, 1135 and 1136 for IGF1R).
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Receptor Tyrosine Kinases
      • Signal Transduction
      • Growth Factors/Hormones
      • Insulin / Insulin-like
      • Neuroscience
      • Neurology process
      • Metabolism
      • Cancer
      • Growth factors
      • Insulin and insulin-like
      • Developmental Biology
      • Post embryonic development
      • Aging
      • Neuroscience
      • Processes

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human IGF1 Receptor protein (ab155622)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab5681于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    1/1000. Detects a band of approximately 100 kDa.
    说明
    WB
    1/1000. Detects a band of approximately 100 kDa.

    靶标

    • 细胞定位

      IGF1 Receptor: Cell membrane. Insulin Receptor: Cell membrane.
    • 数据库链接

      • Entrez Gene: 3480 Human
      • Entrez Gene: 3643 Human
      • Entrez Gene: 16001 Mouse
      • Entrez Gene: 16337 Mouse
      • Entrez Gene: 24954 Rat
      • Entrez Gene: 25718 Rat
      • Omim: 147370 Human
      • Omim: 147670 Human
      • SwissProt: P06213 Human
      • SwissProt: P08069 Human
      • SwissProt: P15208 Mouse
      • SwissProt: Q60751 Mouse
      • SwissProt: P15127 Rat
      • SwissProt: P24062 Rat
      • Unigene: 465744 Human
      • Unigene: 643120 Human
      • Unigene: 714012 Human
      • Unigene: 268003 Mouse
      • Unigene: 275742 Mouse
      • Unigene: 10957 Rat
      • Unigene: 165078 Rat
      • Unigene: 9876 Rat
      see all

    图片

    • Western blot - Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody (ab5681)
      Western blot - Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody (ab5681)
      All lanes : Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody (ab5681) at 1/1000 dilution (2 hours at room temperature in a 3% BSA-TBST buffer)

      Lane 1 : Unstimulated (-), CHO-T transfected with insulin receptor containing vector whole cell extract with 5% BSA TBST buffer overnight at 4°C
      Lanes 2-8 : Insulin stimulated (+), CHO-T transfected with insulin receptor containing vector whole cell extract with 5% BSA TBST buffer overnight at 4°C

      Secondary
      All lanes : goat F(ab’ 2 anti-rabbit IgG HRP conjugate


      Peptide Competition - prior primary antibody incubation:

      1 and 2 - no peptide, 

      3 - non-phosphorylated peptide corresponding to the immunogen,  

      4 -  generic phosphotyrosine-containing peptide,

      5 to 7 - phosphopeptides corresponding to other IR/IGF1R sites,

      8 - phosphopeptide immunogen.

      10% SDS-PAGE transferred to PVDF.

      Method of detection: Pierce SuperSignal method.

      The data show that only the phosphopeptide corresponding to IR/IGF1R [pYpYpY1158/1162/1163] completely blocks the antibody signal, thereby demonstrating the specificty.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (10)

    发表研究结果有使用 ab5681?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5681 被引用在 10 文献中.

    • Lin L  et al. Upregulation of Klotho Aggravates Insulin Resistance in Gestational Diabetes Mellitus Trophoblast Cells. Genet Res (Camb) 2022:1500768 (2022). PubMed: 36325267
    • Huang TW  et al. Regulation of chitosan-mediated differentiation of human olfactory receptor neurons by insulin-like growth factor binding protein-2. Acta Biomater 97:399-408 (2019). PubMed: 31421230
    • Yang R  et al. Treatment of type 2 diabetes mellitus via reversing insulin resistance and regulating lipid homeostasis in vitro and in vivo using cajanonic acid A. Int J Mol Med 42:2329-2342 (2018). PubMed: 30226559
    • Allodi I  et al. Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS. Sci Rep 6:25960 (2016). PubMed: 27180807
    • Khan I  et al. Epithelial atrophy in oral submucous fibrosis is mediated by copper (II) and arecoline of areca nut. J Cell Mol Med 19:2397-412 (2015). WB . PubMed: 26248978
    • Hansen BF  et al. Molecular Characterisation of Long-Acting Insulin Analogues in Comparison with Human Insulin, IGF-1 and Insulin X10. PLoS One 7:e34274 (2012). PubMed: 22590494
    • Ammoun S  et al. The role of insulin-like growth factors signaling in merlin-deficient human schwannomas. Glia 60:1721-33 (2012). WB ; Human . PubMed: 22821509
    • Bouchentouf M  et al. Monocyte derivatives promote angiogenesis and myocyte survival in a model of myocardial infarction. Cell Transplant 19:369-86 (2010). PubMed: 20021736
    • Tanaka J  et al. Foxo1 links hyperglycemia to LDL oxidation and endothelial nitric oxide synthase dysfunction in vascular endothelial cells. Diabetes 58:2344-54 (2009). WB ; Human . PubMed: 19584310
    • Bobulescu IA  et al. Effect of renal lipid accumulation on proximal tubule Na+/H+ exchange and ammonium secretion. Am J Physiol Renal Physiol 294:F1315-22 (2008). PubMed: 18417539

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (placenta)
    Loading amount
    75 µg
    Specification
    placenta
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 07 2009

    Question

    I use three different anti-insulin receptor antibodies against different pho
    sphorylation sites (ab 60946 - phospho Y1361, ab5678 - phosoho Y972 and ab78355 - phospho Y1158. Appr
    oximately two years ago a phospho insulin receptor antibody was offered which recognized the sites Y1158, Y1162, Y1163. Sinc
    e I am interested in phospho sites Y1162, Y1163 of the insulin receptor I wonder if you offer such an antibody? Is the ab5681 anti IGF1receptor Y1158, Y1162, Y1163 specific for the IGF1 or does that antibody also recognize the phosphorylated insulin receptor at site Y1162 and Y1163?
    Thanks in advance.
    Sincerely,
    Janine Krüger

    Read More

    Abcam community

    Verified customer

    Asked on Aug 29 2012

    Answer

    Wenn Sie einen Antikörper suchen ,der nur mit dem IGF1 Rezeptor reagiert , wenn dieser an Y1158 + Y1162 + Y1163 phosphoryliert ist, ist dies der Richtige für Sie. Er reagiert nicht mit dem unmodifizierten Protein, und laut unserem Datenblatt reagiert der Antikörper nur mit dem Protein, wenn es eben an allen drei Phosphorylierungsstellen modifiziert ist.

    Read More

    Abcam Scientific Support

    回复于 Aug 29 2012

    Question

    i'm interested in detecting insulin receptor activation and have been looking at the literature. It is stablished that autophosphorylation of InsR,to undergo activation, takes place at tyr 1146, tyr 1150 and tyr1151. My question is ,why this phosphospecific Ab recognize phorylation at site 1158, 1162 and 1163? is it because the sequence is in different species? arr those sites known to be phosphorylated aswell, when binding of insulin to the receptor? thanks in advance for your time.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 12 2005

    Answer

    We have just received the following information from the source of ab5681: The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1158, 1162 and 1163 of the human insulin receptor (IR) as numbered according to Ebina, et al. (1146, 1150 and 1151 according to Ullrich, et al.). The corresponding residues in the IGF1R are 1131, 1135 and 1136. The sequence is conserved in human, mouse and rat for both the IR and IGF1R.The two relevant papers are:Ebina, Y., et al. (1985) The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling. Cell 40(4):747-758.Ullrich, A., et al. (1985) Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes. Nature 313(6005):756-761.When I look in NCBI, I see that there is a long and a short isoform of this protein. This is why we are listing 1158, 1162 and 1163 in the name (where these phospho sites in the long isoform) as well as 1146, 1150 and 1151 (for the short isoform) within the TDS. I hope this information helps, please do not hesitate to contact us again if you require further assistance,

    Read More

    Abcam Scientific Support

    回复于 Apr 14 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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