Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163)抗体(ab5681)
Key features and details
- Rabbit polyclonal to IGF1 Receptor (phospho Y1131 + Y1135 + Y1136) + Insulin Receptor (phospho Y1158 + Y1162 + Y1163)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163)抗体 -
描述
兔多克隆抗体to IGF1 Receptor (phospho Y1131 + Y1135 + Y1136) + Insulin Receptor (phospho Y1158 + Y1162 + Y1163) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to IGF1 Receptor (phospho Y1158 + Y1162 + W1163).
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阳性对照
- WB: CHO-T (Chinese hamster ovary cell line) whole cell extract transfected with a vector encoding the human insulin receptor and stimulated with insulin.
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常规说明
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1158, 1162 and 1163 of the human insulin receptor (IR) as numbered according to Ebina, et al. (1146, 1150 and 1151 according to Ullrich, et al.). The corresponding residues in the IGF1R are 1131, 1135 and 1136. The sequence is conserved in human, mouse and rat for both the IR and IGF1R.The two relevant papers are:Ebina, Y., et al. (1985) The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling. Cell 40(4):747-758.Ullrich, A., et al. (1985) Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes. Nature 313(6005):756-761. There are a long and a short isoform of this protein. This is why we are listing 1158, 1162 and 1163 in the name (where these phospho sites in the long isoform) as well as 1146, 1150 and 1151 (for the short isoform).The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Insulin Receptor (IR). The final product is generated by affinity chromatography using an IR-derived peptide phosphorylated at tyrosines 1158, 1162 and 1163 (1131, 1135 and 1136 for IGF1R). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 100 kDa.
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| 说明 |
|---|
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WB
1/1000. Detects a band of approximately 100 kDa. |
靶标
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细胞定位
IGF1 Receptor: Cell membrane. Insulin Receptor: Cell membrane. -
数据库链接
- Entrez Gene: 3480 Human
- Entrez Gene: 3643 Human
- Entrez Gene: 16001 Mouse
- Entrez Gene: 16337 Mouse
- Entrez Gene: 24954 Rat
- Entrez Gene: 25718 Rat
- Omim: 147370 Human
- Omim: 147670 Human
see all
图片
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Western blot - Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody (ab5681)All lanes : Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody (ab5681) at 1/1000 dilution (2 hours at room temperature in a 3% BSA-TBST buffer)
Lane 1 : Unstimulated (-), CHO-T transfected with insulin receptor containing vector whole cell extract with 5% BSA TBST buffer overnight at 4°C
Lanes 2-8 : Insulin stimulated (+), CHO-T transfected with insulin receptor containing vector whole cell extract with 5% BSA TBST buffer overnight at 4°C
Secondary
All lanes : goat F(ab’ 2 anti-rabbit IgG HRP conjugatePeptide Competition - prior primary antibody incubation:
1 and 2 - no peptide,
3 - non-phosphorylated peptide corresponding to the immunogen,
4 - generic phosphotyrosine-containing peptide,
5 to 7 - phosphopeptides corresponding to other IR/IGF1R sites,
8 - phosphopeptide immunogen.
10% SDS-PAGE transferred to PVDF.
Method of detection: Pierce SuperSignal method.
The data show that only the phosphopeptide corresponding to IR/IGF1R [pYpYpY1158/1162/1163] completely blocks the antibody signal, thereby demonstrating the specificty.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab5681 被引用在 10 文献中.
- Lin L et al. Upregulation of Klotho Aggravates Insulin Resistance in Gestational Diabetes Mellitus Trophoblast Cells. Genet Res (Camb) 2022:1500768 (2022). PubMed: 36325267
- Huang TW et al. Regulation of chitosan-mediated differentiation of human olfactory receptor neurons by insulin-like growth factor binding protein-2. Acta Biomater 97:399-408 (2019). PubMed: 31421230
- Yang R et al. Treatment of type 2 diabetes mellitus via reversing insulin resistance and regulating lipid homeostasis in vitro and in vivo using cajanonic acid A. Int J Mol Med 42:2329-2342 (2018). PubMed: 30226559
- Allodi I et al. Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS. Sci Rep 6:25960 (2016). PubMed: 27180807
- Khan I et al. Epithelial atrophy in oral submucous fibrosis is mediated by copper (II) and arecoline of areca nut. J Cell Mol Med 19:2397-412 (2015). WB . PubMed: 26248978
- Hansen BF et al. Molecular Characterisation of Long-Acting Insulin Analogues in Comparison with Human Insulin, IGF-1 and Insulin X10. PLoS One 7:e34274 (2012). PubMed: 22590494
- Ammoun S et al. The role of insulin-like growth factors signaling in merlin-deficient human schwannomas. Glia 60:1721-33 (2012). WB ; Human . PubMed: 22821509
- Bouchentouf M et al. Monocyte derivatives promote angiogenesis and myocyte survival in a model of myocardial infarction. Cell Transplant 19:369-86 (2010). PubMed: 20021736
- Tanaka J et al. Foxo1 links hyperglycemia to LDL oxidation and endothelial nitric oxide synthase dysfunction in vascular endothelial cells. Diabetes 58:2344-54 (2009). WB ; Human . PubMed: 19584310
- Bobulescu IA et al. Effect of renal lipid accumulation on proximal tubule Na+/H+ exchange and ammonium secretion. Am J Physiol Renal Physiol 294:F1315-22 (2008). PubMed: 18417539