Anti-IGF1抗体(ab9572)
Key features and details
- Rabbit polyclonal to IGF1
- Suitable for: ICC/IF, IHC-FoFr, WB, Neutralising, IHC-P, Sandwich ELISA
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IGF1抗体
参阅全部 IGF1 一抗 -
描述
兔多克隆抗体to IGF1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-FoFr, WB, Neutralising, IHC-P, Sandwich ELISAmore details -
种属反应性
与反应: Mouse, Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human IGF1. Highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1).
Database link: P05019 -
阳性对照
- IHC-P: Human pancreas and liver tissues.
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常规说明
Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see ab263903
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
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sELISA pair antibody
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
Use at an assay dependent concentration.
|
| IHC-FoFr | (1) |
Use at an assay dependent concentration. PubMed: 28754163
|
| WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required. |
|
| IHC-P | (1) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to IGF1 (ab83137). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1.
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-FoFr
Use at an assay dependent concentration. PubMed: 28754163 |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required. |
|
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to IGF1 (ab83137). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1. |
靶标
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功能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
疾病相关
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
序列相似性
Belongs to the insulin family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3479 Human
- Entrez Gene: 16000 Mouse
- Omim: 147440 Human
- SwissProt: P01343 Human
- SwissProt: P05019 Human
- SwissProt: P05017 Mouse
- Unigene: 160562 Human
- Unigene: 268521 Mouse
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形式
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA. -
别名
- IBP1 antibody
- IGF I antibody
- IGF IA antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab9572)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling IGF1 with ab9572 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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Sandwich ELISA - Anti-IGF1 antibody (ab9572)To detect hIGF-I by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-I as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab9572)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IGF1 with ab9572 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
数据表及文件
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SDS download
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Datasheet download
文献 (104)
ab9572 被引用在 104 文献中.
- Kamaludin Z et al. Angiopoietin-Like Protein 4 and Insulin-Like Growth Factor-1 Expression in Invasive Breast Carcinoma in Young Women. Pathophysiology 29:9-23 (2022). PubMed: 35366286
- Ballester-Rosado CJ et al. A Role for Insulin-like Growth Factor 1 in the Generation of Epileptic Spasms in a murine model. Ann Neurol 92:45-60 (2022). PubMed: 35467038
- Wang X et al. Therapeutic Effects of Retinoic Acid in Lipopolysaccharide-Induced Cardiac Dysfunction: Network Pharmacology and Experimental Validation. J Inflamm Res 15:4963-4979 (2022). PubMed: 36105385
- Park JY et al. The Potential of Transforming Growth Factor-beta Inhibitor and Vascular Endothelial Growth Factor Inhibitor as Therapeutic Agents for Uterine Leiomyoma. Int J Med Sci 19:1779-1786 (2022). PubMed: 36313223
- Takata S et al. LIF-IGF Axis Contributes to the Proliferation of Neural Progenitor Cells in Developing Rat Cerebrum. Int J Mol Sci 23:N/A (2022). PubMed: 36361987