Anti-IgE抗体[5D4] (ab7295)
Key features and details
- Mouse monoclonal [5D4] to IgE
- Suitable for: Sandwich ELISA
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-IgE抗体[5D4]
参阅全部 IgE 一抗 -
描述
小鼠单克隆抗体[5D4] to IgE -
宿主
Mouse -
特异性
Specific for IgE Ce2-domain specific -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human IgE. IgE from myelomatous human serum.
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常规说明
Concentration varies from lot to lot and can be provided on request.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis. -
克隆
单克隆 -
克隆编号
5D4 -
骨髓瘤
Sp2/0 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
Can be used as Capture antibody with recommended pair. |
说明 |
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Sandwich ELISA
Use at an assay dependent concentration. Can be used as Capture antibody with recommended pair. |
靶标
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相关性
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock. -
细胞定位
Cell Membrane -
数据库链接
- Entrez Gene: 3497 Human
- Omim: 147180 Human
- SwissProt: P01854 Human
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别名
- Igh2 antibody
- IGHE antibody
- IGHEP1 antibody
- Immunoglobulin heavy constant epsilon antibody
图片
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Standard curve: Capture anitbody (ab7295) at 5 ug/ml in PBS, human IgE from 100 ng/ml to 1.56 ng/ml, detection antibody (ab7294, HRP conjugated) at 0.7 ug/ml concentration.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab7295 被引用在 1 文献中.
- Nguyen AT et al. DOT1L regulates dystrophin expression and is critical for cardiac function. Genes Dev 25:263-74 (2011). PubMed: 21289070