重组Anti-IFNGR1抗体[EPR24127-14] - BSA and Azide free (Capture) (ab281285)
![Sandwich ELISA - Anti-IFNGR1 antibody [EPR24127-14] - BSA and Azide free (Capture) (ab281285)](https://www.abcam.cn/ps/products/281/ab281285/Images/ab281285-2-mouse-ifngr1-elisa-kit-sandwich-elisa-standard-curve-4.jpg "Sandwich ELISA - Anti-IFNGR1 antibody [EPR24127-14] - BSA and Azide free (Capture) (ab281285)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24127-14] to IFNGR1 - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Mouse
概述
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产品名称
Anti-IFNGR1抗体[EPR24127-14] - BSA and Azide free (Capture)
参阅全部 IFNGR1 一抗 -
描述
兔单克隆抗体[EPR24127-14] to IFNGR1 - BSA and Azide free (Capture) -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment corresponding to Mouse IFNGR1 aa 1-300 (internal sequence).
Database link: P15261 -
常规说明
ab281285 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify mouse IFNGR1. The recommended pair for sandwich ELISA is:
Capture: ab281285, mouse IFNGR1 Capture Antibody (unconjugated)
Detector: ab281135, mouse IFNGR1 Detector Antibody (unconjugated)
The reference range value is 15.6 - 4000 pg/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR24127-14 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281285于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795).
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795). |
靶标
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功能
Receptor for interferon gamma. Two receptors bind one interferon gamma dimer. -
疾病相关
Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. -
序列相似性
Belongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
翻译后修饰
Phosphorylated at Ser/Thr residues. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 15979 Mouse
- SwissProt: P15261 Mouse
- Unigene: 549 Mouse
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别名
- Antiviral Protein Type II antibody
- Antiviral protein, type 2 antibody
- AVP type II antibody
see all
图片
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Sandwich ELISA - Anti-IFNGR1 antibody [EPR24127-14] - BSA and Azide free (Capture) (ab281285)Example of Mouse IFNGR1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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![Anti-IFNGR1 antibody [EPR24127-14] - BSA and Azide free (Capture) (ab281285)](https://www.abcam.cn/ps/products/281/ab281285/Images/ab281285-1-RecombinantAntibodyBenefitfigure.png)
Anti-IFNGR1 antibody [EPR24127-14] - BSA and Azide free (Capture) (ab281285)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab281285 尚未被引用在任何文献中。
