重组Anti-IFN gamma Receptor beta/AF-1抗体[EPR22171] (ab224197)
![Flow Cytometry - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)](https://www.abcam.cn/ps/products/224/ab224197/Images/ab224197-319044-anti-ifn-gamma-receptor-beta-af-1-antibody-epr22171-flow-cytometry.jpg "Flow Cytometry - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22171] to IFN gamma Receptor beta/AF-1
- Suitable for: ELISA, ICC/IF, Flow Cyt
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
概述
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产品名称
Anti-IFN gamma Receptor beta/AF-1抗体[EPR22171]
参阅全部 IFN gamma Receptor beta/AF-1 一抗 -
描述
兔单克隆抗体[EPR22171] to IFN gamma Receptor beta/AF-1 -
宿主
Rabbit -
经测试应用
适用于: ELISA, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct. Flow Cyt: HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22171 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224197于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/500.
|
|
| Flow Cyt |
1/400.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
ICC/IF
1/500. |
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Flow Cyt
1/400. |
靶标
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功能
Part of the receptor for interferon gamma. Required for signal transduction. This accessory factor is an integral part of the IFN-gamma signal transduction pathway and is likely to interact with GAF, JAK1, and/or JAK2. -
疾病相关
Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. -
序列相似性
Belongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3460 Human
- Omim: 147569 Human
- SwissProt: P38484 Human
- Unigene: 634632 Human
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别名
- AF 1 antibody
- AF-1 antibody
- AF1 antibody
see all
图片
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Flow Cytometry - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)
Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct cell line labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/400 dilution.
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with isotype control Rabbit monoclonal IgG (ab172730) (Left panel) or IFN gamma Receptor beta/AF-1 (Right panel), then co-stained with Alexa Fluor® 647 conjugated-Myc-tag antibody.
Gated on viable cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)](https://www.abcam.cn/ps/products/224/ab224197/Images/ab224197-319043-anti-ifn-gamma-receptor-beta-af-1-antibody-epr22171-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining in HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct. The nuclear counterstain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red) and Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (white).
The negative control is the secondary antibody only. -
![ELISA - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)](https://www.abcam.cn/ps/products/224/ab224197/Images/ab224197-3-anti-ifn-gamma-receptor-betaaf-1-antibody-epr22171-elisa-human-ifngr2.jpg)
ELISA - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)ELISA analysis of Human IFNGR2 recombinant protein at 1000 ng/mL with ab224197. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody. -
![Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)](https://www.abcam.cn/ps/products/224/ab224197/Images/ab224197-4-benefits-of-recombinant-antibodies.png)
Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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