重组Anti-IFITM1抗体[EPR22620-12] - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFITM1 antibody [EPR22620-12] - BSA and Azide free (AB255273)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling IFITM1 with ab233545 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood vessels of human cerebrum (PMID : 24603679). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab233545 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233545).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-IFITM1 antibody [EPR22620-12] - BSA and Azide free (AB255273)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) or A549 (human lung carcinoma cell line) cells labeling IFITM1 with ab233545 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in K-562 cells. Negative control : A549 (PMID : 25105503). The nuclear cpounter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

PBS only control : Use PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233545).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFITM1 antibody [EPR22620-12] - BSA and Azide free (AB255273)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IFITM1 with ab233545 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lymphocytes of human tonsil is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab233545 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233545).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-IFITM1 antibody [EPR22620-12] - BSA and Azide free (AB255273)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) (Right) or A549 (human lung carcinoma cell line) (Left) cells labeling IFITM1 with ab233545 at 1/500 (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

Negative control : A549 (PMID : 25105503).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233545).

• IP

Unknown

Immunoprecipitation - Anti-IFITM1 antibody [EPR22620-12] - BSA and Azide free (AB255273)

IFITM1 was immunoprecipitated from 0.35 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab233545 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233545 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : K562 whole cell lysate 10 μg (Input).
Lane 2 : ab233545 IP in K562 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab233545 in K562 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233545).

All lanes:

Immunoprecipitation - Anti-IFITM1 antibody [EPR22620-12] (<a href='/products/primary-antibodies/ifitm1-antibody-epr22620-12-ab233545'>ab233545</a>)

Predicted band size: 13 kDa

Observed band size: 17 kDa

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