Anti-IFITM1抗体

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IFITM1 antibody (AB224063)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for IFITM1 (green) using ab224063 (4 μg/ml) in ICC/IF.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFITM1 antibody (AB224063)

Paraffin embedded human epididymis tissue stained for IFITM1 with ab224063 (1/200 dilution) in immunohistochemical analysis.

• WB

Supplier Data

Western blot - Anti-IFITM1 antibody (AB224063)

All lanes:

Western blot - Anti-IFITM1 antibody (ab224063) at 1/100 dilution

All lanes:

CAPAN-2 cell lysate

Predicted band size: 13 kDa

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• WB

CiteAb

Western blot - Anti-IFITM1 antibody (AB224063)

IFITM1 western blot using anti-IFITM1 antibody ab224063. Publication image and figure legend from Kim, S. H., In Choi, H., et al., 2020, Stem Cell Res Ther, PubMed 31910882.

ab224063 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab224063 please see the product overview.

IFITM1 regulation in cell migration responses. a Effect of I3C on IFITM1 mRNA expression in TLR4-stimulated hMSCs measured by quantitative real-time PCR. Gene expression was normalized to GAPDH transcript levels. The data represent three independent experiments. **p < 0.005. b Effect of I3C on IFITM1 protein expression in TLR4-stimulated hMSCs measured by western blot. Protein expression was normalized to β-ACTIN expression level. c, d Quantitative real-time PCR analysis of IFITM1 mRNA in IRF1 siRNA-treated hMSCs and IFITM1 siRNA-treated cells. Gene expression was normalized to GAPDH transcript levels. **p < 0.005. e Effect of knockdown of IRF1 and IFITM1 on the IFITM1 expression in TLR4-stimulated hMSCs assessed by immunofluorescence microscopy. Original magnification, × 400. f The migration of hMSCs was determined using a wound healing migration assay. Representative images of migrating hMSCs after 24 h in TLR4-stimulated or IFITM1 siRNA-treated hMSCs are shown on the left. On the right, the numbers of cells (%) migrated into the middle blank fields after 24 h of incubation are shown. There was a significant difference between the TLR4-stimulated and IFITM1 siRNA-treated hMSCs in the wound healing migration assay when compared with the TLR4-stimulated hMSCs (**p < 0.005)

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