IFIT1抗体[OTI3G8]| Abcam中文官网

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Western blot - Anti-IFIT1 antibody [OTI3G8] (ab118062)

Key features and details

Mouse monoclonal [OTI3G8] to IFIT1
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Human, African green monkey
Isotype: IgG1

产品名称

Anti-IFIT1抗体[OTI3G8]
参阅全部 IFIT1 一抗
描述

小鼠单克隆抗体[OTI3G8] to IFIT1
宿主

Mouse
经测试应用

适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
种属反应性

与反应: Human, African green monkey
免疫原

Recombinant full length protein corresponding to Human IFIT1. Recombinant full length Human IFIT1 produced in HEK293T cells (NP_000899).
Database link: P09914
阳性对照
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY IFIT1 cDNA. IHC-P: Human liver carcinoma, Human ovary adenocarcinoma, kidney and liver tissues. ICC/IF: HT29 cells and COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 cDNA. Flow Cyt (Intra): HEK293T cells transfected with pCMV6-ENTRY IFIT1 overexpress plasmid
常规说明

The clone number has been updated from 3G8 to OTI3G8, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. The product can be stored for up to 12 months.
存储溶液

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 48% PBS, 1% BSA
Concentration information loading...
纯度

IgG fraction
纯化说明

ab118062 is purified from TCS by affinity chromatography.
克隆

单克隆
克隆编号

OTI3G8
同种型

IgG1
研究领域

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Positive Controls
- Jurkat whole cell lysate (ab7899)
Recombinant Protein
- Recombinant Human IFIT1 protein (ab152468)

The Abpromise guarantee

Abpromise™承诺保证使用ab118062于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB		1/500 - 1/1000. Predicted molecular weight: 55 kDa.
IHC-P		1/50.
ICC/IF		1/50 - 1/100.

说明
Flow Cyt (Intra) 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/1000. Predicted molecular weight: 55 kDa.
IHC-P 1/50.
ICC/IF 1/50 - 1/100.

序列相似性

Belongs to the IFIT family.
Contains 10 TPR repeats.
Target information above from: UniProt accession P09914 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 3434 Human
- Omim: 147690 Human
- SwissProt: P09914 Human
- Unigene: 20315 Human
别名
- C56 antibody
- G10P1 antibody
- GARG 16 antibody
- GARG16 antibody
- IFI 56 antibody
- IFI 56K antibody
- IFI-56K antibody
- IFI56 antibody
- IFIT-1 antibody
- Ifit1 antibody
- IFIT1_HUMAN antibody
- IFNAI1 antibody
- Interferon alpha inducible protein (MW 56kD) antibody
- Interferon induced 56 kDa protein antibody
- Interferon induced protein 56 antibody
- Interferon inducible mRNA 561 antibody
- Interferon-induced 56 kDa protein antibody
- Interferon-induced protein with tetratricopeptide repeats 1 antibody
- ISG56 antibody
- OTTHUMP00000020062 antibody
- P56 antibody
- RNM561 antibody
see all

Western blot - Anti-IFIT1 antibody [OTI3G8] (ab118062)

All lanes : Anti-IFIT1 antibody [OTI3G8] (ab118062) at 1/500 dilution

Lane 1 : HEK293T cell lyaste, transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate, transfected with pCMV6-ENTRY IFIT1 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 55 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203320)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 by Immunofluorescence.
Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in HT29 cells by Immunofluorescence.
Flow Cytometry (Intracellular) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/100 dilution staining IFIT1 in HEK293T cells transfected with either pCMV6-ENTRY IFIT1 overexpress plasmid(Red) or empty vector control plasmid(Blue) by Flow Cytometry (Intracellular).

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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发表研究结果有使用 ab118062？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab118062 被引用在 2 文献中.

Gao Y et al. Fusobacterium nucleatum stimulates cell proliferation and promotes PD-L1 expression via IFIT1-related signal in colorectal cancer. Neoplasia 35:100850 (2023). PubMed: 36371909
Berard AR et al. Comparative proteomic analyses of two reovirus T3D subtypes and comparison to T1L identifies multiple novel proteins in key cellular pathogenic pathways. Proteomics 15:2113-35 (2015). PubMed: 25900405

客户评价及客户问答

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Question

Staining of dog cells was non-specific by ICC and also flow cytometry.

Abcam community

Verified customer

Asked on Oct 12 2012

Answer

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service.

Abcam Scientific Support

回复于 Oct 12 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

纯化说明

克隆

克隆编号

同种型

研究领域

相关产品

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

序列相似性

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (2)

客户评价及客户问答