AB169788

重组Anti-IFI16抗体[EPR11767(B)]

Anti-IFI16 antibody [EPR11767(B)]

RabMAb
Recombinant
20ul selling size
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(14 Publications)

Rabbit Recombinant Monoclonal IFI16 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 14 publications.

查看别名

IFNGIP1, IFI16, Gamma-interferon-inducible protein 16, Ifi-16, Interferon-inducible myeloid differentiation transcriptional activator

10 Images

Flow Cytometry (Intracellular) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Intracellular Flow Cytometry analysis of Raji (human Burkitt's lymphoma) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 with ab169788 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077 Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

The image shows nuclear staining on Jurkat cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human tonsil tissue labeling IFI16 using ab169788 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Immunofluorescence analysis of Raji cells labeling IFI16, using ab169788 at a 1/100 dilution.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

ab169788 showing -ve staining in Human gastric adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human bone marrow tissue labeling IFI16 using ab169788 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

ab169788 showing -ve staining in Human normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

ab169788 showing +ve staining in Human normal spleen tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

All lanes:

Western blot - Anti-IFI16 antibody [EPR11767(B)] (ab169788) at 1/1000 dilution

Lane 1:

U937 treated with IFN gamma cell lysate at 10 µg

Lane 2:

U937 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

Raji cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 88 kDa

true

不同偶联物与剂型 (10)

775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-IFI16 antibody [EPR11767(B)]
Carrier free

Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free
578 PE

PE Anti-IFI16 antibody [EPR11767(B)]
660 APC

APC Anti-IFI16 antibody [EPR11767(B)]
HRP

HRP Anti-IFI16 antibody [EPR11767(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-IFI16 antibody [EPR11767(B)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-IFI16 antibody [EPR11767(B)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-IFI16 antibody [EPR11767(B)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-IFI16 antibody [EPR11767(B)]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-IFI16 antibody [EPR11767(B)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11767(B)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra), IHC-P, WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "The use of an HRP/AP polymerized secondary antibody is recommended.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The IFI16 protein also known as Interferon γ Inducible Protein 16 is a DNA sensor belonging to the PYHIN family with a molecular mass of approximately 85 kDa. Widely expressed in the nucleus and cytoplasm its primary role involves detecting foreign DNA within cells. It possesses a pyrin domain two HIN-200 domains and also plays a role in regulating gene expression particularly in response to pathogen infection. This versatile protein can act in multiple cellular environments and its location within the cell can dictate its function.

Biological function summary

IFI16 protein participates in the innate immune response by recognizing viral and bacterial double-stranded DNA. It forms part of a signaling complex that activates pathogen recognition receptors and induces the production of type I interferons. IFI16 can interact with various DNA sensors and is known for its ability to bind DNA directly providing a defense mechanism against infection. The protein also plays a role in the regulation of transcription which contributes to cell growth and differentiation.

Pathways

IFI16 protein has significant roles in the cGAS-STING and BRCA1 pathways. It interacts with cGAS to regulate the production of cyclic GMP-AMP activating STING and promoting antiviral responses. Additionally IFI16 aligns with BRCA1 dysfunction impacting DNA repair processes. This interaction underlines its involvement in maintaining genomic integrity and modulating the immune responses along with cGAS STING and BRCA1 proteins which are critical in these pathways.

IFI16 protein plays a role in autoimmune diseases and various cancers. In Systemic Lupus Erythematosus (SLE) it can induce inflammation by recognizing self-DNA leading to chronic immune activation. In certain cancers its expression levels correlate with malignancy where it influences tumor progression and response to therapy. In these contexts IFI16 interacts closely with cGAS and BRCA1 proteins highlighting its relevance to disease mechanisms and offering potential therapeutic targets.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors. Necessary to activate the IRF3 signaling cascade during human herpes simplex virus 1 (HHV-1) infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibition of viral immediate-early gene expression and replication. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.. Isoform IFI16-beta. Isoform that specifically inhibits the AIM2 inflammasome (PubMed : 30104205). Binds double-stranded DNA (dsDNA) in the cytoplasm, impeding its detection by AIM2 (PubMed : 30104205). Also prevents the interaction between AIM2 and PYCARD/ASC via its interaction with AIM2, thereby inhibiting assembly of the AIM2 inflammasome (PubMed : 30104205). This isoform also weakly induce production of type I interferon-beta (IFNB1) via its interaction with STING1 (PubMed : 30104205).

See full target information IFI16

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Biology methods & protocols 10:bpaf068 PubMed41018846

2025

A robust protocol for proteomic profiling of secreted proteins in conditioned culture medium.

Applications

Unspecified application

Species

Unspecified reactive species

Takayoshi Otsuka,Atsushi Hatano,Masaki Matsumoto,Hideaki Matsui

Mediators of inflammation 2025:3585885 PubMed40726812

2025

G Protein-Coupled Receptor 30 Attenuates Neuronal Pyroptosis Induced by Subarachnoid Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Peng,Xiqi Hu,Jun He,Ying Xia

Renal failure 47:2479575 PubMed40101924

2025

Determining IFI44 as a key lupus nephritis's biomarker through bioinformatics and immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Tan,Xueyao Wang,Deyou Zhang,Jiahui Wang,Shuxian Wang,Jinyu Yu,Hao Wu

Heliyon 11:e42024 PubMed40028521

2025

Unveiling the prognostic value of ARID3A in breast cancer through bioinformatic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Run Cai,Xu-Gang Sun,Yue Yu,Xin Wang,Xu-Chen Cao,Xiao-Feng Liu

Journal of inflammation research 17:11469-11483 PubMed39735896

2024

Macrophage Infiltration Correlated with IFI16, EGR1 and MX1 Expression in Renal Tubular Epithelial Cells Within Lupus Nephritis-Associated Tubulointerstitial Injury via Bioinformatics Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Tian,Min Tang,Caiming Chen,Yufang Lin,Hong Chen,Yanfang Xu

iMeta 3:e221 PubMed39135698

2024

Cell-type-specific expression analysis of liver transcriptomics with clinical parameters to decipher the cause of intrahepatic inflammation in chronic hepatitis B.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Qian Li,Yuanwang Qiu,Simo Kitanovski,Chen Wang,Chenxia Zhang,Fahong Li,Xiaoguang Li,Zhenfeng Zhang,Lihua Huang,Jiming Zhang,Daniel Hoffmann,Mengji Lu,Hongzhou Lu

iScience 27:110350 PubMed39108722

2024

Heterozygous Gnaq deficiency enhances Ifi202b/IFI16 and NF-κB activation in endothelial cells and exacerbates lupus nephritis pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Yan He,Mengqin Zhang,Jimin Zhang,Wuwei Zhuang,Yuechi Sun,Xing Chen,Hangzhou Fu,Xuanli Tang,Guixiu Shi

The FEBS journal 291:3432-3453 PubMed38676954

2024

PLK1-activating IFI16-STING-TBK1 pathway induces apoptosis of intestinal epithelial cells in patients with intestinal Behçet's syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Fang Bao,Chun-Hui She,Cheng-Cheng Hou,Da-Nian Ji,Dan Hu,Jun Zou,Yan Shen,Lei-Lei Jian,Jian-Fei Cai,Jing-Fen Ye,Dan Luo,Hai-Fen Ma,Jian-Long Guan

Immune network 23:e46 PubMed38188602

2024

IL-17 Imbalance Promotes the Pyroptosis in Immune-Mediated Liver Injury Through STAT3-IFI16 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenfang Xu,Yanan Wang,Changzhong Jin,Weiyang Zhang,Jiangnan Chen,Xuefang Chen,Junli Gao,Junshun Gao,Hong Wang

Nature communications 14:6327 PubMed37816771

2023

NAT10-dependent N-acetylcytidine modification mediates PAN RNA stability, KSHV reactivation, and IFI16-related inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Yan,Jing Zhou,Ziyu Wang,Xiangya Ding,Xinyue Ma,Wan Li,Xuemei Jia,Shou-Jiang Gao,Chun Lu

View all publications

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重组Anti-IFI16抗体[EPR11767(B)]

Anti-IFI16 antibody [EPR11767(B)]

Flow Cytometry (Intracellular) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Flow Cytometry (Intracellular) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (AB169788)

Western blot - Anti-IFI16 antibody [EPR11767(B)] (AB169788)