Anti-IFI16抗体[2E3] (ab55328)
![Western blot - Anti-IFI16 antibody [2E3] (ab55328)](https://www.abcam.cn/ps/products/55/ab55328/Images/ab55328-207674-anti-ifi16-antibody-western-blot.jpg "Western blot - Anti-IFI16 antibody [2E3] (ab55328)")
Key features and details
- Mouse monoclonal [2E3] to IFI16
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, Purification
- Reacts with: Human
- Isotype: IgG2b
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IFI16抗体[2E3]
参阅全部 IFI16 一抗 -
描述
小鼠单克隆抗体[2E3] to IFI16 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cyt, Purificationmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment: FVNGVFEVHK KNVRGEFTYY EIQDNTGKME VVVHGRLTTI NCEEGDKLKL TCFELAPKSG NTGELRSVIH SHIKVIKTRK NKKDILNPDS SMETSPDFFF , corresponding to amino acids 630-730 of Human IFI16
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常规说明
This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4 -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
2E3 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab55328于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 88 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF | (2) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
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| Purification |
Use at an assay dependent concentration. PubMed: 22691496
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| 说明 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 88 kDa. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. |
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Purification
Use at an assay dependent concentration. PubMed: 22691496 |
靶标
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相关性
Interferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells. -
细胞定位
Nucleus. Cytoplasm. Note: Cellular distribution is dependent on the acetylation status of the multipartite nuclear localization signal (NLS); NLS acetylation promotes cytoplasmic localization. -
数据库链接
- Entrez Gene: 3428 Human
- Omim: 147586 Human
- SwissProt: Q16666 Human
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别名
- IFI16 antibody
- Gamma interferon inducible protein 16 antibody
- Gamma-interferon-inducible protein Ifi16 antibody
see all
图片
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Western blot - Anti-IFI16 antibody [2E3] (ab55328)This image is courtesy of an Abreview submitted by Jason DehartAll lanes : Anti-IFI16 antibody [2E3] (ab55328) at 1/1000 dilution
Lane 1 : Human sjsa1 whole cell lysate without siRNA to IFI16
Lane 2 : Human sjsa1 whole cell lysate with siRNA to IFI16
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : IRDye® 680-conjugated Mouse anti-mouse IgG polyclonal at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesThis image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [2E3] (ab55328)](https://www.abcam.cn/ps/products/55/ab55328/Images/ab55328-68552-anti-ifi16-antibody-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [2E3] (ab55328)IFI16 antibody (ab55328) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
This image was generated using the ascites version of the product.
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![Western blot - Anti-IFI16 antibody [2E3] (ab55328)](https://www.abcam.cn/ps/products/55/ab55328/Images/ab55328_1.jpg)
Western blot - Anti-IFI16 antibody [2E3] (ab55328)IFI16 antibody (ab55328) at 1μg/lane + HeLa cell lysate at 25μg/lane.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-IFI16 antibody [2E3] (ab55328)](https://www.abcam.cn/ps/products/55/ab55328/Images/ab55328-68550-anti-ifi16-antibody-flow-cytometry.jpg)
Flow Cytometry - Anti-IFI16 antibody [2E3] (ab55328)Overlay histogram showing Jurkat cells stained with ab55328 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55328, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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Datasheet download
文献 (22)
ab55328 被引用在 22 文献中.
- Wang Z et al. Identification of a Metastasis-Related Protein IFI16 in Esophageal Cancer using a Proteomic Approach. J Cancer 13:1630-1639 (2022). PubMed: 35371314
- Liu L et al. IFI16 induces inflammation in hepatitis B virus-associated glomerulonephritis by regulating the Caspase-1/ IL-1 ß pathway. Diagn Pathol 17:39 (2022). PubMed: 35459254
- Mertens ME & Knipe DM Herpes Simplex Virus 1 Manipulates Host Cell Antiviral and Proviral DNA Damage Responses. mBio 12:N/A (2021). PubMed: 33563816
- Krump NA et al. Merkel Cell Polyomavirus Infection Induces an Antiviral Innate Immune Response in Human Dermal Fibroblasts. J Virol 95:e0221120 (2021). PubMed: 33883226
- Peng T et al. Tissue-Resident-Memory CD8+ T Cells Bridge Innate Immune Responses in Neighboring Epithelial Cells to Control Human Genital Herpes. Front Immunol 12:735643 (2021). PubMed: 34552595
