Anti-IDOL抗体(ab74562)
Key features and details
- Rabbit polyclonal to IDOL
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-IDOL抗体 -
描述
兔多克隆抗体to IDOL -
宿主
Rabbit -
经测试应用
适用于: ELISA, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human IDOL (internal sequence). Immunogen Range:161-210
Database link: Q8WY64 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74562于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| WB | (3) |
1/500 - 1/1000. Predicted molecular weight: 50 kDa.
|
| IHC-P | (1) |
1/50 - 1/100.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
WB
1/500 - 1/1000. Predicted molecular weight: 50 kDa. |
|
IHC-P
1/50 - 1/100. |
靶标
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功能
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. -
组织特异性
Ubiquitously expressed. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 FERM domain.
Contains 1 RING-type zinc finger. -
发展阶段
Expressed in fetal tissues and higher levels were detected in placenta and fetal lung. -
结构域
The RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity. -
翻译后修饰
Autoubiquitinated. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 29116 Human
- Entrez Gene: 218203 Mouse
- Omim: 610082 Human
- SwissProt: Q8WY64 Human
- SwissProt: Q8BM54 Mouse
- Unigene: 484738 Human
- Unigene: 212855 Mouse
- Unigene: 481228 Mouse
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别名
- BM-023 antibody
- BZF1 antibody
- E3 ubiquitin protein ligase MYLIP antibody
see all
图片
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Western blot - Anti-IDOL antibody (ab74562)All lanes : Anti-IDOL antibody (ab74562) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from Jurkat cells
Lane 3 : extracts from Jurkat cells with immunising peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDOL antibody (ab74562)Immunohistochemistry analysis of IDOL in paraffin embedded human brain tissue using 1/50 ab74562.
Left image: untreated sample.
Right image: sample treated with immunising peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab74562 被引用在 11 文献中.
- Guo BC et al. Di-(2-ethylhexyl) Phthalate Limits the Lipid-Lowering Effects of Simvastatin by Promoting Protein Degradation of Low-Density Lipoprotein Receptor: Role of PPARγ-PCSK9 and LXRα-IDOL Signaling Pathways. Antioxidants (Basel) 12:N/A (2023). PubMed: 36830035
- Zhao L et al. Long non-coding RNA TUSC8 inhibits breast cancer growth and metastasis via miR-190b-5p/MYLIP axis. Aging (Albany NY) 12:2974-2991 (2020). PubMed: 32039833
- Ito S et al. CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells. Oncotarget 9:17645-17655 (2018). PubMed: 29707137
- Leitch EK et al. Inhibition of low-density lipoprotein receptor degradation with a cyclic peptide that disrupts the homodimerization of IDOL E3 ubiquitin ligase. Chem Sci 9:5957-5966 (2018). PubMed: 30079210
- Zhao L et al. miR-19b promotes breast cancer metastasis through targeting MYLIP and its related cell adhesion molecules. Oncotarget 8:64330-64343 (2017). PubMed: 28969074