Anti-IDH3G抗体(ab224210)
Key features and details
- Rabbit polyclonal to IDH3G
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-IDH3G抗体
参阅全部 IDH3G 一抗 -
描述
兔多克隆抗体to IDH3G -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Cynomolgus monkey
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免疫原
Recombinant fragment corresponding to Human IDH3G aa 250 to the C-terminus.
Database link: P51553 -
阳性对照
- IHC-P: Human cerebellum tissue. ICC/IF: U-251 MG cells. WB: PC-12, NIH/3T3, RT4, U-251 MG, A431 and NBT-II cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224210于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 43 kDa.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 43 kDa. |
靶标
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序列相似性
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 614145 Cow
- Entrez Gene: 3421 Human
- Entrez Gene: 15929 Mouse
- Entrez Gene: 25179 Rat
- Omim: 300089 Human
- SwissProt: IDH3G Cow
- SwissProt: P51553 Human
- SwissProt: P70404 Mouse
see all -
别名
- H IDHG antibody
- IDH gamma antibody
- IDH3G antibody
see all
图片
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Western blot - Anti-IDH3G antibody (ab224210)All lanes : Anti-IDH3G antibody (ab224210) at 1/250 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Predicted band size: 43 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3G antibody (ab224210)Paraffin embedded human cerebellum tissue stained for IDH3G with ab224210 (1/200 dilution) in immunohistochemical analysis.
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Western blot - Anti-IDH3G antibody (ab224210)All lanes : Anti-IDH3G antibody (ab224210) at 1/250 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Predicted band size: 43 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-IDH3G antibody (ab224210)PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for IDH3G (green) using ab224210 (4 µg/ml) in ICC/IF.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab224210 尚未被引用在任何文献中。