Anti-IDH3G抗体(ab118289)
Key features and details
- Goat polyclonal to IDH3G
- Suitable for: WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-IDH3G抗体
参阅全部 IDH3G 一抗 -
描述
山羊多克隆抗体to IDH3G -
宿主
Goat -
特异性
ab118289 is expected to recognize both reported isoforms of IDH3G (NP_004126.1; NP_777358.1). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat, Human, Pig
预测可用于: Cow, Dog
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免疫原
Synthetic peptide:
DHLKLHSYATSIRK
with a Cysteine residue linker, corresponding to internal sequence amino acids 337-350 of Human IDH3G (NP_004126.1; NP_777358.1). -
阳性对照
- MCF7 lysate; Human, Mouse and Rat heart lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab118289 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118289于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 43 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 43 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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序列相似性
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 614145 Cow
- Entrez Gene: 481081 Dog
- Entrez Gene: 3421 Human
- Entrez Gene: 25179 Rat
- Omim: 300089 Human
- SwissProt: IDH3G Cow
- SwissProt: P51553 Human
- SwissProt: IDH3G Pig
see all -
别名
- H IDHG antibody
- IDH gamma antibody
- IDH3G antibody
see all
图片
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Western blot - Anti-IDH3G antibody (ab118289)All lanes : Anti-IDH3G antibody (ab118289) at 1 µg/ml
Lane 1 : Rat heart lysate
Lane 2 : Pig heart lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 43 kDa -
Western blot - Anti-IDH3G antibody (ab118289)Anti-IDH3G antibody (ab118289) at 0.5 µg/ml + MCF7 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab118289 尚未被引用在任何文献中。