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AB177512

Anti-IDH2抗体

Anti-IDH2 antibody

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(2 Publications)

Rabbit Polyclonal IDH2 antibody. Suitable for ICC, IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IDH2 aa 400-500.

查看别名

IDH, ICD-M, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH2

4 Images
Immunocytochemistry - Anti-IDH2 antibody (AB177512)
  • ICC

Unknown

Immunocytochemistry - Anti-IDH2 antibody (AB177512)

Immunocytochemistry analysis of HeLa cells labelling IDH2 with ab177512 at 1 μg/mL. Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated was used as a secondary antivody at a 1/100 dilution.

Immunoprecipitation - Anti-IDH2 antibody (AB177512)
  • IP

Supplier Data

Immunoprecipitation - Anti-IDH2 antibody (AB177512)

Lane 1 : ab177512 at 1μg/ml labeling IDH2 in 293T whole cell lysate immunoprecipitated using ab177512 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 10 seconds.

.

All lanes:

Immunoprecipitation - Anti-IDH2 antibody (ab177512)

Predicted band size: 50 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody (AB177512)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody (AB177512)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling IDH2 with ab177512 at 1 μg/mL.

Detection : DAB

Western blot - Anti-IDH2 antibody (AB177512)
  • WB

Supplier Data

Western blot - Anti-IDH2 antibody (AB177512)

All lanes:

Western blot - Anti-IDH2 antibody (ab177512) at 0.4 µg/mL

Lane 1:

Whole cell lysate from 293T cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 50 µg

Lane 3:

Whole cell lysate from Jurkat cells at 50 µg

Lane 4:

Whole cell lysate from TCMK-1 cells at 50 µg

Lane 5:

Whole cell lysate from NIH3T3 cells at 50 µg

Predicted band size: 50 kDa

true

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

IHC-P, IP, ICC, WB

applications

免疫原

Recombinant Fragment Protein within Human IDH2 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

P48735

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替代产品

Primary Antibodies

AB212122

PE Anti-IDH2 antibody [EPR7577]

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - PE Anti-IDH2 antibody [EPR7577] (AB212122)

  • 5
  • 1
Primary Antibodies

AB129180

Anti-IDH2 antibody [EPR7576]

20ul selling size|KO Validated|RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-IDH2 antibody [EPR7576] (AB129180)

  • 4
  • 3
Primary Antibodies

AB131263

Anti-IDH2 antibody [EPR7577]

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (AB131263)

  • 5
  • 1
Primary Antibodies

AB246343

Anti-IDH2 antibody [EPR7576] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (AB246343)

  • 0
  • 0
Primary Antibodies

AB230796

Anti-IDH2 antibody [EPR7577] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] - BSA and Azide free (AB230796)

  • 4
  • 1

搭配使用产品

Assay Kits

AB102528

Isocitrate Dehydrogenase Assay Kit (Colorimetric)

Functional Studies - Isocitrate Dehydrogenase Assay Kit (Colorimetric) (AB102528)

  • 4
  • 1
Primary Antibodies

AB233838

Anti-Citrate synthetase antibody [EPR8067] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody [EPR8067] - BSA and Azide free (AB233838)

  • 5
  • 4
Primary Antibodies

AB172964

Anti-IDH1 antibody [EPR12296]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] (AB172964)

  • 5
  • 2
Primary Antibodies

AB128568

Anti-CPT1A antibody [8F6AE9]

BOND RX™ Validated|KO Validated

Western blot - Anti-CPT1A antibody [8F6AE9] (AB128568)

  • 5
  • 5
Primary Antibodies

AB231828

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)

  • 4
  • 6
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1 µg/mL", "ICC-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>" }, "Chimpanzee": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Isocitrate dehydrogenase 2 (IDH2) is an enzyme that converts isocitrate to alpha-ketoglutarate in the citric acid cycle through oxidative decarboxylation. IDH2 also known as NADP-dependent isocitrate dehydrogenase has a molecular weight of about 51 kDa. This protein expresses in the mitochondria serving an important role in cellular energy production and intermediary metabolism. By facilitating this conversion IDH2 impacts cellular respiration and energy balance.
Biological function summary

The IDH2 enzyme facilitates cellular metabolism by producing NADPH critical for biosynthesis and antioxidant defense. It functions within the oxidative decarboxylation of isocitrate providing reducing equivalents to keep cellular redox balance. Although not part of a larger enzymatic complex IDH2 acts synergistically with other metabolic enzymes to maintain cellular biochemical pathways.

Pathways

The IDH2 protein participates in the citric acid cycle and redox homeostasis. IDH2 contributes to the tricarboxylic acid (TCA) pathway coupling with other TCA components such as citrate synthase and aconitase. Within the redox pathway it influences glucose metabolism via its link with enzymes like NADPH oxidase ensuring a steady supply of NADPH for biosynthetic reactions.

The IDH2 protein links to certain cancers like acute myeloid leukemia (AML) due to mutations such as IDH2 R140Q and IDH2 R172K which lead to the production of oncometabolite 2-hydroxyglutarate. This oncometabolite influences epigenetic regulation and cellular differentiation. IDH2 mutations often associate with altered tumor suppressor pathways involving proteins like TP53 contributing to tumorigenesis and impaired cellular differentiation.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Plays a role in intermediary metabolism and energy production (PubMed : 19228619, PubMed : 22416140). It may tightly associate or interact with the pyruvate dehydrogenase complex (PubMed : 19228619, PubMed : 22416140).
See full target information IDH2
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37446160

2023

Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar Sperm.

Applications

Unspecified application

Species

Unspecified reactive species

Malik Ahsan Ali,Ziyue Qin,Shan Dou,Anqi Huang,Yihan Wang,Xiang Yuan,Yan Zhang,Qingyong Ni,Rameesha Azmat,Changjun Zeng

Journal of experimental & clinical cancer research : CR 41:261 PubMed36028854

2022

ALKBH5-mediated m6A modification of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Leilei Liang,Yunshu Zhu,Jian Li,Jia Zeng,Lingying Wu
View all publications
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