Anti-IDH2抗体(ab109588)
Key features and details
- Goat polyclonal to IDH2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-IDH2抗体
参阅全部 IDH2 一抗 -
描述
山羊多克隆抗体to IDH2 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Pig
预测可用于: Rabbit, Horse, Chicken, Cow, Dog, Macaque monkey
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免疫原
Synthetic peptide:
CIHGLSNVKLNE
, corresponding to internal sequence amino acids 418-429 of Human IDH2 according to NP_002159.2 -
阳性对照
- Human, Mouse or Rat Heart lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109588于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. -
疾病相关
D-2-hydroxyglutaric aciduria 2
Glioma
enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. -
序列相似性
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
翻译后修饰
Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 327669 Cow
- Entrez Gene: 3418 Human
- Entrez Gene: 269951 Mouse
- Entrez Gene: 397603 Pig
- Entrez Gene: 361596 Rat
- Omim: 147650 Human
- SwissProt: Q04467 Cow
- SwissProt: P48735 Human
see all -
别名
- D2HGA2 antibody
- ICD-M antibody
- IDH antibody
see all
图片
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Western blot - Anti-IDH2 antibody (ab109588)All lanes : Anti-IDH2 antibody (ab109588) at 0.1 µg/ml
Lane 1 : Mouse heart tissue lysates
Lane 2 : Rat heart tissue lysates
Lane 3 : Pig heart tissue lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 51 kDaPrimary incubation was 1 hour.
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Western blot - Anti-IDH2 antibody (ab109588)Anti-IDH2 antibody (ab109588) at 0.1 µg/ml + Human heart lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab109588 被引用在 1 文献中.
- Feng L et al. Icariside II alleviates oxygen-glucose deprivation and reoxygenation-induced PC12 cell oxidative injury by activating Nrf2/SIRT3 signaling pathway. Biomed Pharmacother 103:9-17 (2018). PubMed: 29635133