AB264058

重组Anti-IDH1 (mutated R132G)抗体[GMab-r1] - BSA and Azide free

Anti-IDH1 (mutated R132G) antibody [GMab-r1] - BSA and Azide free

Recombinant
了解详情

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(1 Publication)

Rat Recombinant Monoclonal IDH1 mutated R132G antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Human samples. Cited in 1 publication.

查看别名

PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

2 Images

Lab

Western blot - Anti-IDH1 (mutated R132G) antibody [GMab-r1] - BSA and Azide free (AB264058)

Western blot - Anti-IDH1 (mutated R132 G) antibody [GMab-r1] (ab264050).

ab264050 used at a 1/1000 dilution (1.266μg/ml) followed by Goat Anti-Rat IgG (H+L), HRP) (ab205720) used at a 1/10,000 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST.

Lane 1 : His-tagged human IDH1 (aa1-414) recombinant protein 10ng
Lane 2 : His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
Lane 3 : His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
Lane 4 : His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
Lane 5 : His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
Lane 6 : His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
Lane 7 : His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
Lane 8 : His-tagged human IDH2 (aa40-452) recombinant protein 10ng
Lane 9 : His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
Lane 10 : His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
Lane 11 : His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
Lane 12 : His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
Lane 13 : His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
Lane 14 : His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264050).

All lanes:

Western blot - Anti-IDH1 (mutated R132G) antibody [GMab-r1] (<a href='/products/primary-antibodies/idh1-mutated-r132g-antibody-gmab-r1-ab264050'>ab264050</a>)

Predicted band size: 46 kDa

false

Exposure time: 3s

Indirect ELISA - Anti-IDH1 (mutated R132G) antibody [GMab-r1] - BSA and Azide free (AB264058)

I-ELISA

Lab

Indirect ELISA - Anti-IDH1 (mutated R132G) antibody [GMab-r1] - BSA and Azide free (AB264058)

ELISA - Anti-IDH1 (mutated R132 G) antibody [GMab-r1] (ab264050).

ab264050 used at 0-5000 ng/ml, followed by an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) secondary used at 1/1000 dilution.
Antigen concentration 100 ng/ml.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264050).

不同偶联物与剂型 (1)

Unconjugated

Anti-IDH1 (mutated R132G) antibody [GMab-r1]

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

GMab-r1

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

Yes

反应种属

Human

应用

I-ELISA, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" } } }

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产品详情

ab264058 is the carrier-free version of ab264050.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Ion exchange chromatography

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

IDH1 also known as isocitrate dehydrogenase 1 is an enzyme with a molecular weight of approximately 45 kDa. It participates in the citric acid cycle aiding the conversion of isocitrate to alpha-ketoglutarate while producing NADPH. IDH1 resides primarily in the cytoplasm and peroxisomes of cells. It finds expression in various tissues most notably the liver and brain where metabolic processes are highly active.

Biological function summary

Isocitrate dehydrogenase 1 plays an important role in cellular metabolism by helping to maintain the NADP+/NADPH balance important for redox reactions. This enzyme operates mainly as a homodimer meaning two identical subunits form a complex for functional activity. It contributes significantly to lipid and glucose metabolism due to its production of NADPH which the cells use for biosynthetic pathways and detoxification.

Pathways

The involvement of IDH1 in the citric acid cycle highlights its importance in energy production and metabolic regulation. This enzyme closely associates with other metabolic enzymes such as IDH2 another member of the isocitrate dehydrogenase family found in mitochondria suggesting a coordinated function between cytoplasmic and mitochondrial metabolism. Beyond its role in the citric acid cycle IDH1 also relates to the pentose phosphate pathway an alternative glucose metabolism route providing reducing equivalents and ribose 5-phosphate for nucleotide synthesis.

IDH1 mutations particularly the IDH1 R132H variant frequently appear in certain cancers like gliomas and acute myeloid leukemia (AML). These mutations lead to a neomorphic enzyme function producing 2-hydroxyglutarate instead of alpha-ketoglutarate disrupting normal cellular metabolism and aiding tumorigenesis. The mutated IDH1 also affects the TET2 protein which involves in DNA demethylation potentially altering gene expression and contributing to oncogenic pathways.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed : 10521434, PubMed : 19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed : 10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).

See full target information IDH1 mutated R132G

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Monoclonal antibodies in immunodiagnosis and immunotherapy 32:224-8 PubMed23750482

2013

Novel monoclonal antibodies GMab-r1 and LMab-1 specifically recognize IDH1-R132G and IDH1-R132L mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Mika Kato Kaneko,Yuta Tsujimoto,Yasukazu Hozumi,Kaoru Goto,Yukinari Kato

View all publications

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-IDH1 (mutated R132G)抗体[GMab-r1] - BSA and Azide free