重组Anti-IDH1抗体[EPR12296] (ab172964)
![Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)](https://www.abcam.cn/ps/products/172/ab172964/Images/ab172964-467538-anti-idh1-antibody-epr12296-westernblot-raji-human.jpg "Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12296] to IDH1
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-IDH1抗体[EPR12296]
参阅全部 IDH1 一抗 -
描述
兔单克隆抗体[EPR12296] to IDH1 -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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经测试应用
适用于: WB, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Sheep, Cow, Orangutan
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HAP1, HepG2, and HeLa cell lysates. IHC-P: Human gastric cancer tissue IP: HepG2 whole cell lysate WB: Raji, HepG2 and Neuro-2a whole cell lysates. Rat kidney lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12296 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
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Substrate reagent
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172964于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
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| IP |
1/10 - 1/100.
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| 说明 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
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IP
1/10 - 1/100. |
靶标
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疾病相关
Glioma
Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. -
序列相似性
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
翻译后修饰
Acetylation at Lys-374 dramatically reduces catalytic activity. -
细胞定位
Cytoplasm. Peroxisome. - Information by UniProt
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数据库链接
- Entrez Gene: 281235 Cow
- Entrez Gene: 3417 Human
- Entrez Gene: 15926 Mouse
- Entrez Gene: 100171634 Orangutan
- Entrez Gene: 24479 Rat
- Entrez Gene: 443257 Sheep
- Omim: 147700 Human
- SwissProt: Q9XSG3 Cow
see all -
别名
- Cytosolic NADP isocitrate dehydrogenase antibody
- Cytosolic NADP-isocitrate dehydrogenase antibody
- Epididymis luminal protein 216 antibody
see all
图片
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Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)Anti-IDH1 antibody [EPR12296] (ab172964) at 1/1000 dilution (Purified) + Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 47 kDa -
![Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)](https://www.abcam.cn/ps/products/172/ab172964/Images/ab172964-467537-anti-idh1-antibody-epr12296-westernblot-human-mouse-rat.jpg)
Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)All lanes : Anti-IDH1 antibody [EPR12296] (ab172964) at 1/1000 dilution (Purified)
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : Rat kidney lysate
Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 47 kDa -
![Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)](https://www.abcam.cn/ps/products/172/ab172964/Images/ab172964-280457-anti-isocitrate-dehydrogenase-antibody-epr12296-western-blot.jpg)
Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: IDH1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab172964 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab172964 was shown to specifically react with IDH1 in wild-type HAP1 cells. No band was observed when IDH1 knockout samples were used. Wild-type and IDH1 knockout samples were subjected to SDS-PAGE. ab172964 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] (ab172964)](https://www.abcam.cn/ps/products/172/ab172964/Images/ab172964-6-anti-idh1-antibody-epr12296-immunohistochemistry-gastric-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] (ab172964)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric cancer tissue sections labeling IDH1 with purified ab172964 at 1/200 dilution (0.60 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. -
![Immunoprecipitation - Anti-IDH1 antibody [EPR12296] (ab172964)](https://www.abcam.cn/ps/products/172/ab172964/Images/ab172964-7-anti-idh1-antibody-epr12296-immunoprecipitation-hepg2-human.jpg)
Immunoprecipitation - Anti-IDH1 antibody [EPR12296] (ab172964)Purified ab172964 at 1/20 dilution (0.6µg) immunoprecipitating IDH1 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate.
Lane 2 (+): ab172964 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab172964 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST. -
![Anti-IDH1 antibody [EPR12296] (ab172964)](https://www.abcam.cn/ps/products/172/ab172964/Images/ab172964-5-benefits-of-recombinant-antibodies.png)
Anti-IDH1 antibody [EPR12296] (ab172964)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab172964 被引用在 18 文献中.
- Syed S et al. Prognostic value of epigenetic markers for canine mast cell cancer. PLoS One 18:e0283616 (2023). PubMed: 36996057
- Li B et al. SIX4 upregulates IDH1 and metabolic reprogramming to promote osteosarcoma progression. J Cell Mol Med 27:259-265 (2023). PubMed: 36601689
- Yuan Y et al. Convolutional neural network to predict IDH mutation status in glioma from chemical exchange saturation transfer imaging at 7 Tesla. Front Oncol 13:1134626 (2023). PubMed: 37223677
- Hu X et al. IDH1 Mutation Induces HIF-1α and Confers Angiogenic Properties in Chondrosarcoma JJ012 Cells. Dis Markers 2022:7729968 (2022). PubMed: 35198082
- Zhang Q et al. miR-145-5p inhibits the proliferation of glioma stem cells by targeting translationally controlled tumor protein. J Cancer 13:1490-1500 (2022). PubMed: 35371310