重组Anti-ID4抗体[EPR22323-36] - BSA and Azide free (ab273062)
![Western blot - Anti-ID4 antibody [EPR22323-36] - BSA and Azide free (ab273062)](https://www.abcam.cn/ps/products/273/ab273062/Images/ab273062-391394-antiid4antibodyepr2232336westernblothumantestistissuelysate20ug.jpg "Western blot - Anti-ID4 antibody [EPR22323-36] - BSA and Azide free (ab273062)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22323-36] to ID4 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ID4抗体[EPR22323-36] - BSA and Azide free
参阅全部 ID4 一抗 -
描述
兔单克隆抗体[EPR22323-36] to ID4 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human testis and ovary tissue lysates; Mouse testis and ovary tissue lysates; Rat testis and ovary tissue lysates; 3T3-L1, NIH/3T3, 293T and F9 whole cell lysates.
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常规说明
ab273062 is the carrier-free version of ab220166.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22323-36 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273062于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa. |
靶标
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功能
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3400 Human
- Entrez Gene: 15904 Mouse
- Entrez Gene: 291023 Rat
- Omim: 600581 Human
- SwissProt: P47928 Human
- SwissProt: P41139 Mouse
- Unigene: 519601 Human
- Unigene: 458006 Mouse
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别名
- bHLHb27 antibody
- Class B basic helix-loop-helix protein 27 antibody
- DNA binding protein inhibitor ID 4 antibody
see all
图片
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Western blot - Anti-ID4 antibody [EPR22323-36] - BSA and Azide free (ab273062)All lanes : Anti-ID4 antibody [EPR22323-36] (ab220166) at 1/1000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Human ovary tissue lysate
Lane 3 : Human liver tissue lysate
Lane 4 : Mouse testis tissue lysate
Lane 5 : Mouse ovary tissue lysate
Lane 6 : Mouse liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver (PMID:8139914).
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab220166).
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![Anti-ID4 antibody [EPR22323-36] - BSA and Azide free (ab273062)](https://www.abcam.cn/ps/products/273/ab273062/Images/ab273062-2-benefits-of-recombinant-antibodies.png)
Anti-ID4 antibody [EPR22323-36] - BSA and Azide free (ab273062)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab273062 尚未被引用在任何文献中。
