Anti-ID2抗体[2457C5a] (ab53545)
![Western blot - Anti-ID2 antibody [2457C5a] (ab53545)](https://www.abcam.cn/ps/products/53/ab53545/Images/ab53545-66424-anti-id2-antibody-2457c5a-western-blot.gif "Western blot - Anti-ID2 antibody [2457C5a] (ab53545)")
Key features and details
- Mouse monoclonal [2457C5a] to ID2
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
-
产品名称
Anti-ID2抗体[2457C5a]
参阅全部 ID2 一抗 -
描述
小鼠单克隆抗体[2457C5a] to ID2 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Recombinant human ID2
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
ab53545 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
克隆
单克隆 -
克隆编号
2457C5a -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53545于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 15 kDa).
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 15 kDa). |
靶标
-
功能
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. -
组织特异性
Highly expressed in early fetal tissues, including those of the central nervous system. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
发展阶段
Found in most early fetal tissues but not in the corresponding mature tissues. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
-
别名
- bHLHb26 antibody
- Cell growth inhibiting gene 8 antibody
- class B basic helix loop helix protein 26 antibody
see all
图片
-
Western blot - Anti-ID2 antibody [2457C5a] (ab53545)Anti-ID2 antibody [2457C5a] (ab53545) + immunised recombinant protein
Predicted band size: 15 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
数据表及文件
-
Datasheet download
文献 (1)
ab53545 被引用在 1 文献中.
- Cao Y et al. ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression. Nat Commun 10:1280 (2019). PubMed: 30894532
