重组Anti-ICOS Ligand/ICOSL抗体[EPR6071] (ab124972)
![Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)](https://www.abcam.cn/ps/products/124/ab124972/Images/ab124972-139061-anti-icos-ligand-antibody-epr6071-western-blot.jpg "Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6071] to ICOS Ligand/ICOSL
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ICOS Ligand/ICOSL抗体[EPR6071]
参阅全部 ICOS Ligand/ICOSL 一抗 -
描述
兔单克隆抗体[EPR6071] to ICOS Ligand/ICOSL -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human ICOS Ligand/ICOSL aa 250-350 (intracellular). The exact sequence is proprietary.
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阳性对照
- HL60, HuT78 and HUVEC (untreated or treated with TNF-alpha) lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 8.80 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6071 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124972于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 63 - 72 kDa (predicted molecular weight: 33 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 63 - 72 kDa (predicted molecular weight: 33 kDa). |
应用说明
Is unsuitable for Flow Cyt,IHC-P or IP.
靶标
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功能
Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. -
组织特异性
Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells. -
序列相似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 23308 Human
- Omim: 605717 Human
- SwissProt: O75144 Human
- Unigene: 14155 Human
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别名
- B7 H2 antibody
- B7 homolog 2 antibody
- B7 homologue 2 antibody
see all
图片
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Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : HuT78 cell lysate
Lane 3 : HUVEC cell lysate, treated with TNF-alpha
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa -
![OI-RD Scanning - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)](https://www.abcam.cn/ps/products/124/ab124972/Images/ab124972-139060-anti-icos-ligand-antibody-epr6071.jpg)
OI-RD Scanning - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972) -
![Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)](https://www.abcam.cn/ps/products/124/ab124972/Images/ab124972-3-benefits-of-recombinant-antibodies.png)
Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab124972 被引用在 1 文献中.
- Tao YW et al. Association between Increased Inducible Costimulator/Inducible Costimulator Ligand Expression with Bone Destruction in Apical Periodontitis. J Endod 45:890-897 (2019). PubMed: 31153660