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重组RabMAb

重组Anti-ICOS抗体[SP98] (ab105227)

Reviews (2)Q&A (1)References (14)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)
  • Immunohistochemistry (Frozen sections) - Anti-ICOS antibody [SP98] (ab105227)
  • Immunohistochemistry (Frozen sections) - Anti-ICOS antibody [SP98] (ab105227)
  • Anti-ICOS antibody [SP98] (ab105227)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP98] to ICOS
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Carrier Free

概述

  • 产品名称

    Anti-ICOS抗体[SP98]
    参阅全部 ICOS 一抗

  • 描述

    兔单克隆抗体[SP98] to ICOS

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, IHC-Frmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide within Human ICOS aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9Y6W8

  • 阳性对照

    • IHC-P: Human lymph node, pancreatic adenocarcinoma, thymus and tonsil tissue. IHC-Fr: Frozen human tonsil tissue sections

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • 纯度

    Protein A/G purified

  • 纯化说明

    Purified from TCS by protein A/G.

  • 克隆

    单克隆

  • 克隆编号

    SP98

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab105227于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at RT for 20 min.
IHC-Fr (1)
1/100.
说明
IHC-P
1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at RT for 20 min.
IHC-Fr
1/100.

靶标

  • 功能

    Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes.

  • 组织特异性

    Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.

  • 疾病相关

    Immunodeficiency, common variable, 1

  • 序列相似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 翻译后修饰

    N-glycosylated.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Activation inducible lymphocyte immunomediatory molecule antibody
      • Activation-inducible lymphocyte immunomediatory molecule antibody
      • AILIM antibody
      • CD278 antibody
      • CD278 antigen antibody
      • CD28-related Protein-1 antibody
      • CRP1 antibody
      • CVID1 antibody
      • ICOS antibody
      • ICOS_HUMAN antibody
      • Inducible costimulator antibody
      • Inducible T cell co stimulator antibody
      • Inducible T-cell costimulator antibody
      • MGC39850 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling ICOS with ab105227 at 1/100 dilution (0.28 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 30 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadically positive staining in the germinal center of human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab105227 for 30 mins at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for ICOS using ab105227 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)
      ab105227, at 1/100 dilution, staining ICOS in formalin-fixed, paraffin-embedded Human reactive lymph node tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)

      Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for ICOS using ab105227 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)

      Formalin-fixed, paraffin-embedded human thymus tissue stained for ICOS using ab105227 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Frozen sections) - Anti-ICOS antibody [SP98] (ab105227)
      Immunohistochemistry (Frozen sections) - Anti-ICOS antibody [SP98] (ab105227)

      IHC image of ICOS staining in a section of frozen normal human tonsil* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab105227, 5ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.


      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Frozen sections) - Anti-ICOS antibody [SP98] (ab105227)
      Immunohistochemistry (Frozen sections) - Anti-ICOS antibody [SP98] (ab105227)
      Negative control image: IHC image of ICOS staining in a section of frozen normal human placenta* performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab105227, 5ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
    • Anti-ICOS antibody [SP98] (ab105227)
      Anti-ICOS antibody [SP98] (ab105227)

    文献 (14)

    发表研究结果有使用 ab105227？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab105227 被引用在 14 文献中.

    • Donzel M  et al. Deciphering the spectrum of cutaneous lymphomas expressing TFH markers. Sci Rep 13:6500 (2023). PubMed: 37081015
    • Samson C  et al. Belatacept inhibit human B cell germinal center development in immunodeficient mice. Sci Rep 13:13816 (2023). PubMed: 37620431
    • Abraham R  et al. Specific in situ inflammatory states associate with progression to renal failure in lupus nephritis. J Clin Invest 132:N/A (2022). PubMed: 35608910
    • Wang J  et al. Transcriptome profile and clinical characterization of ICOS expression in gliomas. Front Oncol 12:946967 (2022). PubMed: 36276141
    • Pontarini E  et al. Unique expansion of IL-21+ Tfh and Tph cells under control of ICOS identifies Sjögren's syndrome with ectopic germinal centres and MALT lymphoma. Ann Rheum Dis 79:1588-1599 (2020). PubMed: 32963045
    View all Publications for this product

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ICOS antibody [SP98]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Leica BOND ER2
    Permeabilization
    No
    Specification
    tonsil
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Masahiro Hirata

    Verified customer

    提交于 Aug 26 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-ICOS antibody [SP98]

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Spleen)
    Specification
    Spleen
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 29 2012

    Question

    I would like to test ab105227 using IHC-Frozen mouse tissue.

    Read More
    Answer

    DISCOUNT CODE: xxx
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab105227 in IHC-Fr and mouse. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-Fr and mouseand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com