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重组RabMAb

重组Anti-ICAM3抗体[EPR3994-123] (ab109405)

Submit a review Submit a question References (1)
Western blot - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
  • Immunocytochemistry/ Immunofluorescence - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
  • Anti-ICAM3 antibody [EPR3994-123] (ab109405)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3994-123] to ICAM3
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-ICAM3抗体[EPR3994-123]
    参阅全部 ICAM3 一抗

  • 描述

    兔单克隆抗体[EPR3994-123] to ICAM3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • U937, HeLa, Jurkat, HuT-78, Ramos, and THP-1 cell lysates; Human spleen, colon and tonsil tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab109405于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100 - 1/250.
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
ICC/IF
1/100 - 1/250.
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能

    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.

  • 序列相似性

    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

  • 翻译后修饰

    Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
    N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD50 antibody
      • CDW 50 antibody
      • CDw50 antibody
      • ICAM 3 antibody
      • ICAM-3 antibody
      • ICAM-R antibody
      • ICAM3 antibody
      • ICAM3_HUMAN antibody
      • ICAMR antibody
      • Intercellular adhesion molecule 3 antibody
      see all

    图片

    • Western blot - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Western blot - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      All lanes : Anti-ICAM3 antibody [EPR3994-123] (ab109405) at 1/1000 dilution

      Lane 1 : U937 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HuT-78 cell lysate
      Lane 4 : Ramos cell lysate
      Lane 5 : THP-1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 60 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

      Immunohistochemical analysis of ICAM3 in paraffin-embedded Human spleen tissue using ab109405 at 1/500 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Immunocytochemistry/ Immunofluorescence - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      This data was developed using the same antibody clone in a different buffer formulation (ab247851).
      Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) labeling ICAM3 with purified ab247851 at 1/500 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.24 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
      Negative control: PBS instead of the primary antibody.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

      Immunohistochemical analysis of ICAM3 in paraffin-embedded Human colon tissue using ab109405 at 1/500 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

      Immunohistochemical analysis of ICAM3 in paraffin-embedded Human tonsil tissue using ab109405 at 1/500dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Anti-ICAM3 antibody [EPR3994-123] (ab109405)

    文献 (1)

    发表研究结果有使用 ab109405？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab109405 被引用在 1 文献中.

    • Shen W  et al. ICAM3 mediates tumor metastasis via a LFA-1-ICAM3-ERM dependent manner. Biochim Biophys Acta 1864:2566-2578 (2018). WB . PubMed: 29729315

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