Anti-ICAM1抗体[MEM-111] (ab2213)
Key features and details
- Mouse monoclonal [MEM-111] to ICAM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-ICAM1抗体[MEM-111]
参阅全部 ICAM1 一抗 -
描述
小鼠单克隆抗体[MEM-111] to ICAM1 -
宿主
Mouse -
特异性
his antibody reacts with human CD54 antigen.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human ICAM1. Burkitt's lymphoma cell line RAJI
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阳性对照
- For WB it is highly recommended to use TNF-alpha activated HUVEC cells as a positive control.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
MEM-111 -
骨髓瘤
unknown -
同种型
IgG2a -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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sELISA pair antibody
应用
应用 | Ab评论 | 说明 |
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WB | (3) |
Use at an assay dependent concentration. Use under non-reducing conditions.
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说明 |
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WB
Use at an assay dependent concentration. Use under non-reducing conditions. |
靶标
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功能
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
序列相似性
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
翻译后修饰
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
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别名
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
图片
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All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213)
Lane 1 : TNF-alpha activated HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : TNF-alpha nonactivated HUVEC cells
Performed under non-reducing conditions.
Observed band size: 90 kDa why is the actual band size different from the predicted?Lower bands represent tubulin as a loading control.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (72)
ab2213 被引用在 72 文献中.
- Harris AR et al. Platinum Chemotherapy Induces Lymphangiogenesis in Cancerous and Healthy Tissues That Can be Prevented With Adjuvant Anti-VEGFR3 Therapy. Front Oncol 12:801764 (2022). PubMed: 35372032
- Lim EJ et al. ICAM-1 promotes cancer progression by regulating SRC activity as an adapter protein in colorectal cancer. Cell Death Dis 13:417 (2022). PubMed: 35487888
- Sznurkowski JJ et al. Impact of Activation of EGFL7 within Microenvironment of High Grade Ovarian Serous Carcinoma on Infiltration of CD4+ and CD8+ Lymphocytes. Medicina (Kaunas) 58:N/A (2022). PubMed: 35630004
- Ding S et al. ICAM-1-related noncoding RNA accelerates atherosclerosis by amplifying NF-κB signaling. J Mol Cell Cardiol 170:75-86 (2022). PubMed: 35714558
- van den Braak WJP et al. Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines. Viruses 14:N/A (2022). PubMed: 36298792