重组Anti-ICAM1抗体[EPR16608] (ab179707)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16608] to ICAM1
- Suitable for: IHC-P, WB
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-ICAM1抗体[EPR16608]
参阅全部 ICAM1 一抗 -
描述
兔单克隆抗体[EPR16608] to ICAM1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse spleen, liver, kidney and lung lysates; bEnd.3, A20, RAW 264.7 and WEHI-231 whole cell lysates. IHC-P: Mouse liver and kidney tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR16608 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab179707于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (3) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
WB | (1) |
1/1000. Detects a band of approximately 80-110 kDa (predicted molecular weight: 58 kDa).
|
说明 |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 80-110 kDa (predicted molecular weight: 58 kDa). |
靶标
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功能
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
序列相似性
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
翻译后修饰
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 15894 Mouse
- SwissProt: P13597 Mouse
- Unigene: 435508 Mouse
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别名
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling ICAM1 with ab179707 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Positive staining on endothelial cells of mouse liver is observed [PMID: 17058232].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ICAM1 with ab179707 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Positive staining on endothelial cells of mouse kidney is observed [PMID: 17058232].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-ICAM1 antibody [EPR16608] (ab179707) at 1/1000 dilution
Lane 1 : Mouse spleen lysate at 20 µg
Lane 2 : Mouse liver lysate at 20 µg
Lane 3 : Mouse kidney lysate at 20 µg
Lane 4 : bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate at 10 µg
Lane 5 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 80-110 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The observed molecular weight is consistent with the literature (PMID: 1712683).
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All lanes : Anti-ICAM1 antibody [EPR16608] (ab179707) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 80-110 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2: 1 second; Lane 3: 10 seconds.
The observed molecular weight is consistent with the literature (PMID: 1712683).
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (46)
ab179707 被引用在 46 文献中.
- Rojas GA et al. Modulation of the Cardiovascular Effects of Polycyclic Aromatic Hydrocarbons: Physical Exercise as a Protective Strategy. Toxics 11:N/A (2023). PubMed: 37888695
- Gao S et al. Endothelial SARS-CoV-2 infection is not the underlying cause of COVID-19-associated vascular pathology in mice. Front Cardiovasc Med 10:1266276 (2023). PubMed: 37823176
- Xiao M et al. Skin Wound following Irradiation Aggravates Radiation-Induced Brain Injury in a Mouse Model. Int J Mol Sci 24:N/A (2023). PubMed: 37445879
- Ye M et al. Adenosine A2A receptor controls the gateway of the choroid plexus. Purinergic Signal 19:135-144 (2023). PubMed: 35167016
- Fan X et al. PM2.5 increases susceptibility to acute exacerbation of COPD via NOX4/Nrf2 redox imbalance-mediated mitophagy. Redox Biol 59:102587 (2023). PubMed: 36608590