AB108521

重组Anti-ICAD抗体[EPR4222(2)]

Anti-ICAD antibody [EPR4222(2)]

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal ICAD antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

查看别名

DFF1, DFF45, H13, DFFA, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, Inhibitor of CAD, DFF-45, ICAD

2 Images

Lab

Western blot - Anti-ICAD antibody [EPR4222(2)] (AB108521)

Western blot : Anti-ICAD antibody [EPR4222(2)] ab108521 staining at 1/10000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 40 kDa in Wild-type A549 cell lysates with no signal observed at this size in DFFA CRISPR-Cas9 Edited A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-ICAD antibody [EPR4222(2)] (ab108521) at 1/10000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

DFFA CRISPR-Cas9 Edited A549 at 20 µg

Lane 3:

PC-3 at 20 µg

Lane 4:

HeLa at 20 µg

Lane 5:

THP-1 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa,50 kDa

false

Unknown

Western blot - Anti-ICAD antibody [EPR4222(2)] (AB108521)

All lanes:

Western blot - Anti-ICAD antibody [EPR4222(2)] (ab108521) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

U937 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Predicted band size: 37 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-ICAD antibody [EPR4222(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4222(2)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

储存信息

Stable for 12 months at -20°C

style

left-column

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Inhibitor of the caspase-activated DNase (DFF40).

See full target information DFFA

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer drug resistance (Alhambra, Calif.) 2:335-350 PubMed35582720

2019

Daunorubicin can eliminate iPS-derived cancer stem cells via ICAD/CAD-independent DNA fragmentation.

Applications

Unspecified application

Species

Unspecified reactive species

Akimasa Seno,Akifumi Mizutani,Kazuki Aizawa,Ryoma Onoue,Junko Masuda,Naotaka Ochi,Saki Taniguchi,Tatsuyuki Sota,Yuki Hiramoto,Taisuke Michiue,Neha Nair,Masaharu Seno

Experimental and therapeutic medicine 14:6183-6192 PubMed29250143

2017

Molecular mechanisms underlying the α-tomatine-directed apoptosis in human malignant glioblastoma cell lines A172 and U-118 MG.

Applications

Unspecified application

Species

Unspecified reactive species

Fa-Zhao Wang,Xue-Liang Dai,Hong-Yi Liu

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-ICAD抗体[EPR4222(2)]