Anti-Iba1 抗体

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody - Microglia marker (AB108539)

IHC image of Iba1 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody - Microglia marker (AB108539)

ab108539, at 1/100 dilution, staining Iba1 in formalin-fixed, paraffin-embedded Human fetal lymphocytes by Immunohistochemistry.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody - Microglia marker (AB108539)

IHC image of Iba1 staining in normal mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody - Microglia marker (AB108539)

IHC image of Iba1 staining in normal rat brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

Lab

Western blot - Anti-Iba1 antibody - Microglia marker (AB108539)

Lanes 1-14 : Blocked in 3% milk for 1 hour (RT). Lanes 15-16 : Blocked in 5% BSA for 1 hour (RT).

Abcam recommends blocking in milk for cleaner blots with reduced background, in comparison to BSA.

This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab108539 (anti-Iba1 antibody; 1/1000) for 18 hours at 4°C. Antibody binding was detected using HRP-labelled anti-Rabbit IgG for 1 hour at room temperature and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Iba1 antibody - Microglia marker (ab108539) at 1/1000 dilution

Lane 1:

Human Iba1 recombinant protein at 0.1 µg

Lane 2:

HEK293 whole cell lysate at 20 µg

Lane 3:

A431 whole cell lysate at 20 µg

Lane 4:

NIH3T3 whole cell lysate at 30 µg

Lane 5:

Human spleen tissue lysate at 20 µg

Lane 6:

Mouse spleen tissue lysate at 30 µg

Lane 7:

Rat spleen tissue lysate at 30 µg

Lane 8:

U937 whole cell lysate at 30 µg

Lane 9:

MOLT4 whole cell lysate at 20 µg

Lane 10:

THP1 whole cell lysate at 30 µg

Lane 11:

THP1 whole cell lysate, PMA treated at 30 µg

Lane 12:

Raw 264.7 whole cell lysate at 30 µg

Lane 13:

C6 whole cell lysate at 30 µg

Lane 14:

NR8383 whole cell lysate at 30 µg

Lane 15:

NIH3T3 whole cell lysate - BLOCKED IN 5% BSA at 30 µg

Lane 16:

Human spleen tissue lysate - BLOCKED IN 5% BSA at 20 µg

Predicted band size: 16 kDa

true

Exposure time: 3min

• WB

Unknown

Western blot - Anti-Iba1 antibody - Microglia marker (AB108539)

All lanes:

Western blot - Anti-Iba1 antibody - Microglia marker (ab108539) at 1/500 dilution

All lanes:

Rat brain lysate

Predicted band size: 16 kDa

false