Anti-Iba1抗体(ab5076)
Key features and details
- Goat polyclonal to Iba1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Iba1抗体
参阅全部 Iba1 一抗 -
描述
山羊多克隆抗体to Iba1 -
宿主
Goat -
特异性
ab5076 is expected to recognize the isoforms represented by NP_001614 and NP_116573 but not NP_004838.
Although we received several excellent papers on ab5076 working with rat IHC-P, rat is not a batch tested species for IHC-P and we cannot guarantee.
-
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat, Human
预测可用于: Pig, Macaque monkey
-
免疫原
-
阳性对照
- WB: Human lymph node and rat brain tissue lysate. IHC-P: Human spleen tissue.
-
常规说明
The Iba1 antibody (ab5076) is commonly used as a marker of microglia activation in staining and immunohistochemistry, given that ionized calcium binding adaptor molecule 1 (Iba1) is a microglia/macrophage-specific calcium-binding protein with actin-bundling activity that participates in membrane ruffling and phagocytosis in activated microglia.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (28) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Although we received several excellent papers on ab5076 working with rat IHC-P, rat is not a batch tested species for IHC-P and we cannot guarantee. |
| WB | (7) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 16-17 kDa (predicted molecular weight: 17 kDa).
A 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background. |
| 说明 |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Although we received several excellent papers on ab5076 working with rat IHC-P, rat is not a batch tested species for IHC-P and we cannot guarantee. |
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 16-17 kDa (predicted molecular weight: 17 kDa). A 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background. |
靶标
-
功能
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. -
组织特异性
Detected in T-lymphocytes and peripheral blood mononuclear cells. -
序列相似性
Contains 2 EF-hand domains. -
翻译后修饰
Phosphorylated on serine residues. -
细胞定位
Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. - Information by UniProt
-
数据库链接
- Entrez Gene: 199 Human
- Entrez Gene: 397271 Pig
- Entrez Gene: 29427 Rat
- Omim: 601833 Human
- SwissProt: P55008 Human
- SwissProt: P81076 Pig
- SwissProt: P55009 Rat
- Unigene: 76364 Human
see all -
别名
- AIF 1 antibody
- AIF-1 antibody
- Aif1 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Iba1 with ab5076 at 8µg/ml.
Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)Negative control: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Iba1 with no primary antibody.
Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
-
Western blot - Anti-Iba1 antibody (ab5076)All lanes : Anti-Iba1 antibody (ab5076) at 1 µg/ml
Lane 1 : Human lymph node tissue lysate in RIPA buffer
Lane 2 : Rat brain tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 17 kDaDetected by chemiluminescence.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)Image from Iris Margalit Trutzer et al, ACTA neuropathologica communications, 7, 40, Fig. 7; doi: 10.1186/s40478-019-0684-8Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lateral prefrontal cortex tissue labeling iba1 labeled microglia (labeled with black arrows) within the cortex using ab5076 at 1/1000 dilution. (Cropped image).
Sections were rinsed in 0.01 m PBS, pH 7.4, followed by 10% serum matching the species of the secondary antibody, 5% bovine serum albumin, and 0.1% Triton X-100 in 0.01 m PBS blocking solution for 1 h and incubated for 2 days at 4 °C in primary antibody. The sections were rinsed in PBS and incubated overnight at 4 °C with a horse anti-Goat IgG (Biotinylated) secondary antibody.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1354)
ab5076 被引用在 1354 文献中.
- Miao X et al. SARM1 Promotes Neurodegeneration and Memory Impairment in Mouse Models of Alzheimer's Disease. Aging Dis 15:390-407 (2024). PubMed: 37307837
- Chen R et al. Thioredoxin1 Binding Metastasis-Associated Lung Adenocarcinoma Transcript 1 Attenuates Inflammation and Apoptosis after Intracerebral Hemorrhage. Aging Dis 15:1384-1397 (2024). PubMed: 37196136
- Adzic Bukvic M et al. Expression of functionally distinct ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in experimental autoimmune encephalomyelitis and neuroinflammatory conditions in vitro. Glia 72:19-33 (2024). PubMed: 37646205
- Wang J et al. Interleukin-4 Modulates Neuroinflammation by Inducing Phenotypic Transformation of Microglia Following Subarachnoid Hemorrhage. Inflammation 47:390-403 (2024). PubMed: 37898992
- Zhang Z et al. Integrated Analysis of Chromatin and Transcriptomic Profiling Identifies PU.1 as a Core Regulatory Factor in Microglial Activation Induced by Chronic Cerebral Hypoperfusion. Mol Neurobiol 61:2569-2589 (2024). PubMed: 37917300