重组Anti-I-FABP抗体[EPR7136] (ab128860)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7136] to I-FABP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-I-FABP抗体[EPR7136]
参阅全部 I-FABP 一抗 -
描述
兔单克隆抗体[EPR7136] to I-FABP -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human I-FABP aa 1-100. The exact sequence is proprietary.
Database link: P12104 -
阳性对照
- WB: Human, Rat, and Mouse small intestine lysates. IHC-P: Human, Rat, and Mouse small intestine tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解离常数(KD)
KD = 2.90 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7136 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128860于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Predicted molecular weight: 15 kDa.
For unpurified use at 1/1000 - 1/10000. |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000. Predicted molecular weight: 15 kDa. For unpurified use at 1/1000 - 1/10000. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. -
组织特异性
Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum. -
序列相似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
结构域
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 2169 Human
- Entrez Gene: 14079 Mouse
- Entrez Gene: 25598 Rat
- Omim: 134640 Human
- SwissProt: P12104 Human
- SwissProt: P55050 Mouse
- SwissProt: P02693 Rat
- Unigene: 282265 Human
see all -
别名
- FABP 2 antibody
- FABP2 antibody
- FABPI antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-I-FABP antibody [EPR7136] (ab128860)Immunohistochemical analysis of paraffin-embedded Human small intestine labeling I-FABP with ab128860 at 1/1000 dilution (0.098 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the small intestine of human. The section was incubated with ab128860 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-I-FABP antibody [EPR7136] (ab128860)Immunohistochemical analysis of paraffin-embedded Mouse small intestine labeling I-FABP with ab128860 at 1/1000 dilution (0.098 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the small intestine of mouse. The section was incubated with ab128860 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-I-FABP antibody [EPR7136] (ab128860)Immunohistochemical analysis of paraffin-embedded Rat small intestine labeling I-FABP with ab128860 at 1/1000 dilution (0.098 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the small intestine of rat. The section was incubated with ab128860 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-I-FABP antibody [EPR7136] (ab128860)Immunohistochemical analysis of paraffin-embedded Human tonsil labeling I-FABP with ab128860 at 1/1000 dilution (0.098 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on the human tonsil. The section was incubated with ab128860 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
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All lanes : Anti-I-FABP antibody [EPR7136] (ab128860) at 1/10000 dilution (Purified)
Lane 1 : Human small intestine lysates
Lane 2 : Mouse small intestine lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST
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Anti-I-FABP antibody [EPR7136] (ab128860) at 1/1000 dilution (Purified) + Rat small intestine lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab128860 被引用在 3 文献中.
- Chamseddine D et al. The mitochondrial UPR regulator ATF5 promotes intestinal barrier function via control of the satiety response. Cell Rep 41:111789 (2022). PubMed: 36516750
- Guo J et al. Metformin-Induced Changes of the Coding Transcriptome and Non-Coding RNAs in the Livers of Non-Alcoholic Fatty Liver Disease Mice. Cell Physiol Biochem 45:1487-1505 (2018). PubMed: 29466788
- Costa DK et al. Reduced intestinal lipid absorption and body weight-independent improvements in insulin sensitivity in high-fat diet-fed Park2 knockout mice. Am J Physiol Endocrinol Metab 311:E105-16 (2016). PubMed: 27166280