Anti-I-FABP抗体(ab60272)
Key features and details
- Goat polyclonal to I-FABP
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, ELISA
- Reacts with: Cat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-I-FABP抗体
参阅全部 I-FABP 一抗 -
描述
山羊多克隆抗体to I-FABP -
宿主
Goat -
经测试应用
适用于: WB, ICC/IF, IHC-P, Flow Cyt, ELISAmore details -
种属反应性
与反应: Cat, Human
预测可用于: Rat, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
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免疫原
Synthetic peptide corresponding to Human I-FABP aa 121-132 (C terminal).
Sequence:C-EGVEAKRIFKKD
(Peptide available asab115238) -
阳性对照
- WB: Human duodenum and ileum lysates. IHC-P: Human small intestine tissue. Flow Cyt: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab60272于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.001 - 0.003 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).
A 1 hour primary incubation is recommended for this product. |
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 23236492
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| IHC-P | (1) |
Use a concentration of 2 - 3 µg/ml.
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| Flow Cyt |
Use a concentration of 10 µg/ml.
|
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| ELISA |
Use at an assay dependent concentration.
Antibody detection limit dilution 1:64000. |
| 说明 |
|---|
|
WB
Use a concentration of 0.001 - 0.003 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa). A 1 hour primary incubation is recommended for this product. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 23236492 |
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IHC-P
Use a concentration of 2 - 3 µg/ml. |
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Flow Cyt
Use a concentration of 10 µg/ml. |
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ELISA
Use at an assay dependent concentration. Antibody detection limit dilution 1:64000. |
靶标
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功能
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. -
组织特异性
Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum. -
序列相似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
结构域
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 2169 Human
- Entrez Gene: 25598 Rat
- Omim: 134640 Human
- SwissProt: Q56JX9 Cow
- SwissProt: P12104 Human
- SwissProt: Q45KW7 Pig
- SwissProt: Q56G53 Pig
- SwissProt: P02693 Rat
see all -
别名
- FABP 2 antibody
- FABP2 antibody
- FABPI antibody
see all
图片
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Western blot - Anti-I-FABP antibody (ab60272)Lane 1 : Anti-I-FABP antibody (ab60272) at 0.001 µg/ml
Lane 2 : Anti-I-FABP antibody (ab60272) at 0.003 µg/ml
Lane 1 : Human Duodenum lysate in RIPA buffer
Lane 2 : Ileum lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 15 kDa
Observed band size: 15-16 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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Flow Cytometry - Anti-I-FABP antibody (ab60272)ab60272 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-I-FABP antibody (ab60272)ab60272 (2.5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab60272 被引用在 5 文献中.
- Baumgarten HD et al. The EXTrauterine Environment for Neonatal Development Supports Normal Intestinal Maturation and Development. Cell Mol Gastroenterol Hepatol 10:623-637 (2020). PubMed: 32474164
- Reicher N et al. It's All About Timing: Early Feeding Promotes Intestinal Maturation by Shifting the Ratios of Specialized Epithelial Cells in Chicks. Front Physiol 11:596457 (2020). PubMed: 33391016
- Tian M et al. Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport. Animals (Basel) 11:N/A (2020). PubMed: 33375218
- Ziegler AL et al. Epithelial restitution defect in neonatal jejunum is rescued by juvenile mucosal homogenate in a pig model of intestinal ischemic injury and repair. PLoS One 13:e0200674 (2018). PubMed: 30138372
- Treutlein G et al. Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis. PLoS One 7:e51391 (2012). WB, ICC/IF ; Cat . PubMed: 23236492