AB96297

Anti-HUS1抗体

Name: HUS1抗体 (ab96297)| Abcam中文官网
Brand: Abcam Limited
SKU: AB96297
Price: 3775 CNY
Availability: InStock

Anti-HUS1 antibody

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(4 Publications)

Rabbit Polyclonal HUS1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HUS1 aa 1-250.

查看别名

Checkpoint protein HUS1, hHUS1, HUS1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HUS1 antibody (AB96297)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HUS1 antibody (AB96297)

ab96297 at 1/500 dilution, staining HUS1 in paraffin-embedded CA922 xenograft.

Unknown

Western blot - Anti-HUS1 antibody (AB96297)

12% SDS PAGE

All lanes:

Western blot - Anti-HUS1 antibody (ab96297) at 1/1000 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 32 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human HUS1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

O60921

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HUS1 also known as checkpoint protein HUS1 homolog is a protein that plays an important role in DNA damage response. It has a molecular mass of about 34 kDa and is expressed in various tissues with notable expression in testis and thymus. HUS1 is part of the Rad9-Rad1-Hus1 (9-1-1) complex which acts as a sensor for DNA damage and activates downstream signaling pathways for DNA repair mechanisms.

Biological function summary

HUS1 is important in maintaining genomic stability and integrity. It forms a complex with RAD9 and RAD1 creating the 9-1-1 complex that localizes to sites of DNA damage. This complex enables the recruitment and activation of several key DNA repair proteins facilitating the repair process. HUS1 plays a role in the activation of cell cycle checkpoints essential for preventing the propagation of damaged DNA during cell division.

Pathways

HUS1 is significantly involved in the DNA damage response and cell cycle regulation pathways. The protein connects into the ATR signaling pathway where it helps activate ATR kinase in response to DNA damage. Related proteins in these pathways include ATM and CHK1 which also serve in coordinating the cellular response to DNA injuries.

HUS1 is associated with cancer pathogenesis particularly in regard to its role in DNA repair. Mutations or dysregulation in HUS1 can lead to genomic instability a hallmark of cancerous cells. In addition to cancer HUS1 plays a role in hereditary diseases such as Ataxia-telangiectasia due to its connection with the ATM protein which is mutated in this disorder. Understanding HUS1's function and interactions might provide insight into therapeutic approaches for these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed : 21659603). The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex (PubMed : 21659603). Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed : 21659603). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates (PubMed : 21659603). The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase (PubMed : 21659603).

See full target information HUS1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical medicine 11: PubMed35456300

2022

HUS1 as a Potential Therapeutic Target in Urothelial Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Katharina Lindner,Tobias Furlan,Jacob J Orme,Gennadi Tulchiner,Nina Staudacher,David D'Andrea,Zoran Culig,Renate Pichler

Cancer cell 35:519-533.e8 PubMed30889383

2019

DNA Replication Vulnerabilities Render Ovarian Cancer Cells Sensitive to Poly(ADP-Ribose) Glycohydrolase Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Nisha Pillay,Anthony Tighe,Louisa Nelson,Samantha Littler,Camilla Coulson-Gilmer,Nourdine Bah,Anya Golder,Bjorn Bakker,Diana C J Spierings,Dominic I James,Kate M Smith,Allan M Jordan,Robert D Morgan,Donald J Ogilvie,Floris Foijer,Dean A Jackson,Stephen S Taylor

PloS one 10:e0144434 PubMed26658951

2015

Chk1 Activation Protects Rad9A from Degradation as Part of a Positive Feedback Loop during Checkpoint Signalling.

Applications

Unspecified application

Species

Unspecified reactive species

William F Osorio-Zambrano,Scott Davey

FEBS letters 587:930-5 PubMed23454212

2013

HIF1-regulated ATRIP expression is required for hypoxia induced ATR activation.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Ding,He-Dai Liu,Hong-Xiang Liang,Ru-Feng Ni,Zhao-Yan Ding,Guo-Ying Ni,Hong-Wei Hua,Wei-Guo Xu

View all publications

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Anti-HUS1抗体

Anti-HUS1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HUS1 antibody (AB96297)

Western blot - Anti-HUS1 antibody (AB96297)