AB238528

Anti-HuR / ELAVL1抗体

Name: HuR / ELAVL1抗体 (ab238528)| Abcam中文官网
Brand: Abcam Limited
SKU: AB238528
Price: 4863 CNY
Availability: InStock

Anti-HuR / ELAVL1 antibody

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(5 Publications)

Rabbit Polyclonal HuR / ELAVL1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ELAVL1 aa 1 to C-terminus.

查看别名

HUR, ELAVL1, ELAV-like protein 1, Hu-antigen R, HuR

14 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human gastric cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human esophagus tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human colon tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human kidney tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human kidney cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human esophageal cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded human stomach tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded rat cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded rat testis tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded rat brain tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded mouse testis tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Paraffin-embedded mouse kidney tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-HuR / ELAVL1 antibody (AB238528)

Blocking buffer : 3% non-fat dry milk in TBST.

All lanes:

Western blot - Anti-HuR / ELAVL1 antibody (ab238528) at 1/1000 dilution

Lane 1:

U-251 MG (Human brain glioma cell line) cell extracts at 25 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts at 25 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell extracts at 25 µg

Lane 4:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell extracts at 25 µg

Lane 5:

PC-3 (Human prostate adenocarcinoma cell line) cell extracts at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-HuR / ELAVL1 antibody (AB238528)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-HuR / ELAVL1 antibody (ab238528) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 25 µg

Lane 2:

HuR / ELAVL1 knockout HEK-293 cell lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 36 kDa

true

Exposure time: 90s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human ELAVL1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15717

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HuR also known as ELAVL1 is a protein that binds RNA in cells. It weighs about 36 kDa and is found in the nucleus and cytoplasm. Its name HuR comes from its similarity to human antigen R. Researchers often call it the HuR protein or ELAVL1 protein. This protein helps stabilize certain mRNA molecules by binding to AU-rich elements (AREs) in their 3' untranslated regions. Its binding affects mRNA decay rates and therefore influences protein synthesis.

Biological function summary

HuR influences cell growth differentiation and stress responses. It does not work alone; it often forms part of larger ribonucleoprotein complexes. By stabilizing mRNAs HuR ensures the continuous production of proteins necessary for cell survival and adaptation to stressors. This regulatory role highlights its involvement in many cellular processes making it a focus for study in relation to cellular homeostasis.

Pathways

HuR is part of the mRNA surveillance pathway and the response to cellular stress. HuR modulates gene expression by interacting with proteins involved in transcription and translation regulation. It frequently partners with proteins like TTP (tristetraprolin) that also bind to AREs affecting mRNA stability and translation processes. Such interactions extend HuR's influence across pathways related to gene expression regulation and response to stress.

Researchers associate HuR with cancer and neurodegenerative diseases. In cancer HuR stabilizes mRNAs of many oncogenes aiding tumor growth and progression. It may interact with proteins like p53 affecting tumor suppressor pathways. For neurodegenerative diseases HuR's role in mRNA stability can influence neural health connected to proteins like TDP-43 in conditions such as Alzheimer's and ALS. Understanding HuR's function offers insights into potential therapeutic targets for these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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靶点信息

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed : 14517288, PubMed : 18285462, PubMed : 31358969). Involved in embryonic stem cell (ESC) differentiation : preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity). Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed : 32245947). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed : 14731398, PubMed : 17632515, PubMed : 18285462, PubMed : 23519412, PubMed : 8626503). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed : 8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed : 29180010).

See full target information ELAVL1

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:33763 PubMed41028834

2025

Icariin inhibits proliferation and migration of VSMCs via LncRNA H19 competitively binding to HuR.

Applications

Unspecified application

Species

Unspecified reactive species

Yibing Zhang,Peng Huang,Min Li,Zhimin Fan

International journal of molecular medicine 56: PubMed40937577

2025

CDC5L binds to ELAVL1 to inhibit pyroptosis in hepatocellular carcinoma through the Caspase 3/GSDME signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Liang,Zhongcheng Zhu,Yangshuo Tang,Shuhua Zhou,Moyan Xiao,Xuejun Gong,Ke Ye

Genes 14: PubMed37510387

2023

Functional Characteristics of Diverse Mutations Associated with Isolated Foveal Hypoplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Itsuka Matsushita,Hiroto Izumi,Shinji Ueno,Takaaki Hayashi,Kaoru Fujinami,Kazushige Tsunoda,Takeshi Iwata,Yoshiaki Kiuchi,Hiroyuki Kondo

Journal of experimental & clinical cancer research : CR 41:185 PubMed35624501

2022

SOCS7/HuR/FOXM1 signaling axis inhibited high-grade serous ovarian carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhua Du,Xiang Xu,Siang Lv,Han Liu,Hong Sun,Jun Wu

Cancer cell international 21:271 PubMed34020639

2021

Circ_0000745 strengthens the expression of CCND1 by functioning as miR-488 sponge and interacting with HuR binding protein to facilitate the development of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kuangzheng Li,Xiaosheng Fan,Ziyi Yan,Jia Zhan,Fangyun Cao,Yixia Jiang

View all publications

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Anti-HuR / ELAVL1抗体

Anti-HuR / ELAVL1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (AB238528)

Western blot - Anti-HuR / ELAVL1 antibody (AB238528)

Western blot - Anti-HuR / ELAVL1 antibody (AB238528)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (5)

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