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Neuroscience Neurology process Neurodegenerative disease Huntington's disease

Anti-Huntingtin (phospho S421)抗体(ab2174)

本 快线抗体 仍未被充份测试和鉴定, 不在质保范围内, 具体请参见 使用条款及细节。
  • Datasheet
  • SDS
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Western blot - Anti-Huntingtin (phospho S421) antibody (ab2174)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Huntingtin (phospho S421) antibody (ab2174)

Key features and details

  • Rabbit polyclonal to Huntingtin (phospho S421)
  • Suitable for: IHC-P, WB, ELISA
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Huntingtin (phospho S421)抗体
    参阅全部 Huntingtin 一抗
  • 描述

    兔多克隆抗体to Huntingtin (phospho S421)

    该产品是 快线抗体,通过亲和纯化得到。对抗原肽有高灵敏度及特异性(ELISA测试)。 Read the terms of use »

  • 宿主

    Rabbit
  • 特异性

    Detects a 190kDa band in PC-3 cells, which may correspond to Huntingtin. Stains neuronal cytoplasm in immunohistochemistry (the expected localisation). The antibody has not been conclusively tested for specificity for phospho S421 Huntingtin.
  • 种属反应性

    与反应: Human
    预测可用于: Pig
  • 免疫原

    Synthetic peptide corresponding to Human Huntingtin aa 400-500 (phospho S421).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Huntingtin protein (Tagged) (ab112300)

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 348 kDa.
ELISA
Use at an assay dependent concentration. This antibody gave a positive result in ELISA against the immunizing peptide .
说明
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 348 kDa.
ELISA
Use at an assay dependent concentration. This antibody gave a positive result in ELISA against the immunizing peptide .

靶标

  • 功能

    May play a role in microtubule-mediated transport or vesicle function.
  • 组织特异性

    Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
  • 疾病相关

    Defects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).
  • 序列相似性

    Belongs to the huntingtin family.
    Contains 10 HEAT repeats.
  • 结构域

    The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.
  • 翻译后修饰

    Cleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
    Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.
  • 细胞定位

    Cytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons.
  • Target information above from: UniProt accession P42858 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3064 Human
    • Omim: 143100 Human
    • Omim: 613004 Human
    • SwissProt: P42858 Human
    • Unigene: 518450 Human
    • 别名

      • AI256365 antibody
      • C430023I11Rik antibody
      • HD antibody
      • HD protein antibody
      • HD_HUMAN antibody
      • HDH antibody
      • HTT antibody
      • Huntingtin antibody
      • HUNTINGTON CHOREA antibody
      • Huntington disease protein antibody
      • Huntington's disease protein homolog antibody
      • IT 15 antibody
      • IT15 antibody
      • OTTMUSP00000026909 antibody
      • ZHD antibody
      see all

    图片

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • Western blot - Anti-Huntingtin (phospho S421) antibody (ab2174)
      Western blot - Anti-Huntingtin (phospho S421) antibody (ab2174)
      Anti-Huntingtin (phospho S421) antibody (ab2174) at 1/1000 dilution + human PC-3 cell lysate

      Predicted band size: 348 kDa
      Observed band size: 190 kDa why is the actual band size different from the predicted?
      Additional bands at: 120 kDa, 130 kDa. We are unsure as to the identity of these extra bands.



      The 190kDa band may correspond to Huntingtin. However, it is not clear whether the antibody is specific for the phospho S421 form.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Huntingtin (phospho S421) antibody (ab2174)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Huntingtin (phospho S421) antibody (ab2174)
      ab2174 was used at a 1:100 dilution to detect phosphorylated Huntingtin by immunohistochemistry in human brain cerebellum. Positive cytoplasmic staining is observed in neurons. Tissue was formalin-fixed and paraffin embedded. Detection was with AEC (pink), nuclear counterstaining with Haemotoxylin (blue).

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab2174?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2174 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Immunohistochemistry (Frozen sections) abreview for Anti-Huntingtin (phospho S421) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 1% Triton X-100 in TBS
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Marian Hruska-Plochan

    Verified customer

    提交于 Oct 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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