Anti-Huntingtin (phospho S421)抗体(ab2174)
Key features and details
- Rabbit polyclonal to Huntingtin (phospho S421)
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Huntingtin (phospho S421)抗体
参阅全部 Huntingtin 一抗 -
描述
兔多克隆抗体to Huntingtin (phospho S421)该产品是 快线抗体，通过亲和纯化得到。对抗原肽有高灵敏度及特异性（ELISA测试）。 Read the terms of use »
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宿主
Rabbit -
特异性
Detects a 190kDa band in PC-3 cells, which may correspond to Huntingtin. Stains neuronal cytoplasm in immunohistochemistry (the expected localisation). The antibody has not been conclusively tested for specificity for phospho S421 Huntingtin.
This antibody is specific for phosphorylated human Huntington protein at the pS421 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Huntington homologues from chimpanzee, pig and chicken. Cross reactivity with Huntington protein homologues from mouse and rat may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Huntington protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
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种属反应性
与反应: Human
预测可用于: Pig
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免疫原
Synthetic peptide corresponding to Human Huntingtin aa 400-500 (phospho S421).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Anti-Huntingtin pS421 is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Huntington protein at the pS421 residue. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| 应用 | Ab评论 | 说明 |
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| IHC-P |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 348 kDa.
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| ELISA |
Use at an assay dependent concentration. This antibody gave a positive result in ELISA against the immunizing peptide .
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| 说明 |
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IHC-P
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 348 kDa. |
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ELISA
Use at an assay dependent concentration. This antibody gave a positive result in ELISA against the immunizing peptide . |
靶标
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功能
May play a role in microtubule-mediated transport or vesicle function. -
组织特异性
Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. -
疾病相关
Defects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum). -
序列相似性
Belongs to the huntingtin family.
Contains 10 HEAT repeats. -
结构域
The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations. -
翻译后修饰
Cleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation. -
细胞定位
Cytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons. - Information by UniProt
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数据库链接
- Entrez Gene: 3064 Human
- Omim: 143100 Human
- Omim: 613004 Human
- SwissProt: P42858 Human
- Unigene: 518450 Human
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别名
- AI256365 antibody
- C430023I11Rik antibody
- HD antibody
see all
图片
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Western blot - Anti-Huntingtin (phospho S421) antibody (ab2174)Anti-Huntingtin (phospho S421) antibody (ab2174) at 1/1000 dilution + human PC-3 cell lysate
Predicted band size: 348 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 130 kDa. We are unsure as to the identity of these extra bands.
The 190kDa band may correspond to Huntingtin. However, it is not clear whether the antibody is specific for the phospho S421 form. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Huntingtin (phospho S421) antibody (ab2174)ab2174 was used at a 1:100 dilution to detect phosphorylated Huntingtin by immunohistochemistry in human brain cerebellum. Positive cytoplasmic staining is observed in neurons. Tissue was formalin-fixed and paraffin embedded. Detection was with AEC (pink), nuclear counterstaining with Haemotoxylin (blue).
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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