重组抗人IgM抗体[EPR5539-65-4] (ab134159)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5539-65-4] to Human IgM
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
抗人IgM抗体[EPR5539-65-4]
参阅全部 Human IgM 一抗 -
描述
兔单克隆抗体[EPR5539-65-4] to人IgM -
宿主
Rabbit -
特异性
This antibody only recognizes the mu chain of IgM.
-
经测试应用
适用于: WB, IP, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- IHC: Human tonsil tissue. WB: Raji cells; IP: Raji cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5539-65-4 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134159于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 75 kDa.
|
|
IP |
1/10 - 1/100.
|
|
IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 75 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
相关性
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. -
细胞定位
Cell Membrane and Secreted -
数据库链接
- Entrez Gene: 3507 Human
- Omim: 147020 Human
- SwissProt: P01871 Human
-
别名
- Immunoglobin heavy chain constant region mu antibody
- AGM1 antibody
- Constant region of heavy chain of IgM antibody
see all
图片
-
Anti-Human IgM antibody [EPR5539-65-4] (ab134159) at 1/1000 dilution (Purified) + Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking Buffer and concentration: 5% NFDM/TBST
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgM antibody [EPR5539-65-4] (ab134159)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling Human IgM with Purified ab134159 at 1:1000 dilution (0.16 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
purifiedab134159 at 1/50 dilution (2µg) immunoprecipitating Human IgM in Raji whole cell lysate.
Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab134159 + Raji whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134159 in Raji whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 75 kDa -
Purified ab134159 at 1/20 dilution (1µg) immunoprecipitating Human IgM in Raji whole cell lysate.
Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab134159 + Raji whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134159 in Raji whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 75 kDa
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (7)
ab134159 被引用在 7 文献中.
- Zheng X et al. Generation of inactivated IL2RG and RAG1 monkeys with severe combined immunodeficiency using base editing. Signal Transduct Target Ther 8:327 (2023). PubMed: 37661226
- Melani RD et al. Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire. J Proteome Res 21:274-288 (2022). PubMed: 34878788
- Melani RD et al. Next-generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire. medRxiv N/A:N/A (2021). PubMed: 34268518
- Du B et al. Increased Circulating T Follicular Helper Cells Induced via IL-12/21 in Patients With Acute on Chronic Hepatitis B Liver Failure. Front Immunol 12:641362 (2021). PubMed: 33868273
- Sánchez-Rodríguez C et al. Immunomodulatory effect of Polypodium leucotomos (Anapsos) in child palatine tonsil model. Int J Pediatr Otorhinolaryngol 107:56-61 (2018). PubMed: 29501312
- Chatterjee D et al. Prevalence of polyreactive innate clones among graft--infiltrating B cells in human cardiac allograft vasculopathy. J Heart Lung Transplant 37:385-393 (2018). PubMed: 29055600
- Lu Y et al. A case of acantholysis related to the internal fixation of an orthopedic steel plate acantholysis related to the bone plate. J Cutan Pathol 43:290-2 (2016). PubMed: 26469404