重组抗人IgG3抗体[EPR4419] (ab109761)
![Western blot - Anti-Human IgG3 antibody [EPR4419] (ab109761)](https://www.abcam.cn/ps/products/109/ab109761/Images/ab109761-122772-anti-human-igg3-antibody-epr4419-western-blot.jpg "Western blot - Anti-Human IgG3 antibody [EPR4419] (ab109761)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4419] to Human IgG3
- Suitable for: WB, IP
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
概述
-
产品名称
抗人IgG3抗体[EPR4419]
参阅全部 Human IgG3 一抗 -
描述
兔单克隆抗体[EPR4419] to人IgG3 -
宿主
Rabbit -
特异性
ab109761 does not cross-react with human IgG1, IgG2, and IgG4. -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- IgG3 recombinant protein, Human tonsil and Human plasma lysates.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4419 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109761于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000.
|
|
| IP |
1/10 - 1/100.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. |
|
IP
1/10 - 1/100. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
-
相关性
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
细胞定位
Secreted -
数据库链接
- Entrez Gene: 3502 Human
- Omim: 147120 Human
- SwissProt: P01860 Human
-
别名
- DKFZp686H11213 antibody
- FLJ39988 antibody
- FLJ40036 antibody
see all
图片
-
Western blot - Anti-Human IgG3 antibody [EPR4419] (ab109761)All lanes : Anti-Human IgG3 antibody [EPR4419] (ab109761) at 1/1000 dilution
Lane 1 : IgG3 at 0.01 µg
Lane 2 : IgG1 at 0.01 µg
Lane 3 : IgG2 at 0.01 µg
Lane 4 : IgG4 recombinant protein at 0.01 µg
Lane 5 : Human tonsil lysate at 10 µg
Lane 6 : Human plasma lysate -
![Anti-Human IgG3 antibody [EPR4419] (ab109761)](https://www.abcam.cn/ps/products/109/ab109761/Images/ab109761-2-benefits-of-recombinant-antibodies.png)
Anti-Human IgG3 antibody [EPR4419] (ab109761)
数据表及文件
-
SDS download
-
Datasheet download
文献 (11)
ab109761 被引用在 11 文献中.
- Melani RD et al. Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire. J Proteome Res 21:274-288 (2022). PubMed: 34878788
- Zhang F et al. Hsa_circ_0010235 functions as an oncogenic drive in non-small cell lung cancer by modulating miR-433-3p/TIPRL axis. Cancer Cell Int 21:73 (2021). PubMed: 33494763
- Liu J et al. Circ_0105346 Knockdown Inhibits Osteosarcoma Development via Regulating miR-1182/WNT7B Axis. Cancer Manag Res 13:521-535 (2021). PubMed: 33505171
- Liu Y et al. MicroRNA-124 facilitates lens epithelial cell apoptosis by inhibiting SPRY2 and MMP-2. Mol Med Rep 23:N/A (2021). PubMed: 33760112
- Meng X et al. Silencing of the Long Non-Coding RNA TTN-AS1 Attenuates the Malignant Progression of Osteosarcoma Cells by Regulating the miR-16-1-3p/TFAP4 Axis. Front Oncol 11:652835 (2021). PubMed: 34141611