AB109761

Anti-Human IgG3 抗体 [EPR4419]

Anti-Human IgG3 antibody [EPR4419]

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(11 Publications)

Rabbit Recombinant Monoclonal IGHG3 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 11 publications.

查看别名

Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3

1 Images

Unknown

Western blot - Anti-Human IgG3 antibody [EPR4419] (AB109761)

All lanes:

Western blot - Anti-Human IgG3 antibody [EPR4419] (ab109761) at 1/1000 dilution

Lane 1:

IgG3 at 0.01 µg

Lane 2:

IgG1 at 0.01 µg

Lane 3:

IgG2 at 0.01 µg

Lane 4:

IgG4 recombinant protein at 0.01 µg

Lane 5:

Human tonsil lysate at 10 µg

Lane 6:

Human plasma lysate

Predicted band size: 41 kDa

false

不同偶联物与剂型 (2)

Carrier free

Anti-Human IgG3 antibody [EPR4419] - BSA and Azide free
Biotin

Biotin Anti-Human IgG3 antibody [EPR4419]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4419

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

ab109761 does not cross-react with human IgG1, IgG2, and IgG4.

style

left-column

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Recombinant fragment - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

style

left-column

产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

style

left-column

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGHG3

style

left-column

文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Journal of proteome research 21:274-288 PubMed34878788

2021

Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael D Melani,Benjamin J Des Soye,Jared O Kafader,Eleonora Forte,Michael Hollas,Voislav Blagojevic,Fernanda Negrão,John P McGee,Bryon Drown,Cameron Lloyd-Jones,Henrique S Seckler,Jeannie M Camarillo,Philip D Compton,Richard D LeDuc,Bryan Early,Ryan T Fellers,Byoung-Kyu Cho,Basil Baby Mattamana,Young Ah Goo,Paul M Thomas,Michelle K Ash,Pavan P Bhimalli,Lena Al-Harthi,Beverly E Sha,Jeffrey R Schneider,Neil L Kelleher

medRxiv : the preprint server for health sciences : PubMed34268518

2021

Next-generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire.

Applications

Unspecified application

Species

Unspecified reactive species

Frontiers in oncology 11:652835 PubMed34141611

2021

Silencing of the Long Non-Coding RNA TTN-AS1 Attenuates the Malignant Progression of Osteosarcoma Cells by Regulating the miR-16-1-3p/TFAP4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xianghai Meng,Zhenjun Zhang,Lin Chen,Xi Wang,Qingguo Zhang,Shuheng Liu

Molecular medicine reports 23: PubMed33760112

2021

MicroRNA-124 facilitates lens epithelial cell apoptosis by inhibiting SPRY2 and MMP-2.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Shuting Li,Yao Liu,Xujing Lv,Qing Zhou

Cancer cell international 21:73 PubMed33494763

2021

Hsa_circ_0010235 functions as an oncogenic drive in non-small cell lung cancer by modulating miR-433-3p/TIPRL axis.

Applications

Unspecified application

Species

Unspecified reactive species

Furui Zhang,Ruirui Cheng,Ping Li,Chunya Lu,Guojun Zhang

Cancer management and research 13:521-535 PubMed33505171

2021

Circ_0105346 Knockdown Inhibits Osteosarcoma Development via Regulating miR-1182/WNT7B Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinbao Liu,Xiaoyang Li,Liang Yue,Hao Lv

Cell cycle (Georgetown, Tex.) 19:3289-3302 PubMed33131409

2020

Depletion of circ_0007841 inhibits multiple myeloma development and BTZ resistance via miR-129-5p/JAG1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Quande Lin,Chunge Song,Ruojin Ma,Xiaojie Li

Molecular medicine reports 22:5095-5104 PubMed33174038

2020

Potential role of lncRNA HULC/miR‑128‑3p/RAC1 axis in the inflammatory response during LPS‑induced sepsis in HMEC‑1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Weize Yang,Xiaomin Luo,Yu Liu,Jun Xiong,Hongxia Xia,Yafeng Liu

Open life sciences 15:683-695 PubMed33817257

2020

CircHIPK3/miR-381-3p axis modulates proliferation, migration, and glycolysis of lung cancer cells by regulating the AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Gu,Junhan Zhang,Lin Yan,Dong Li

Nature immunology 19:1001-1012 PubMed30104633

2018

IgG3 regulates tissue-like memory B cells in HIV-infected individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Lela Kardava,Haewon Sohn,Christine Youn,James W Austin,Wei Wang,Clarisa M Buckner,J Shawn Justement,Valerie A Melson,Gwynne E Roth,Marissa A Hand,Kathleen R Gittens,Richard W Kwan,Michael C Sneller,Yuxing Li,Tae-Wook Chun,Peter D Sun,Susan K Pierce,Susan Moir

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Human IgG3 抗体 [EPR4419]