Anti-Human IgG 抗体 [EPR4421]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (AB109489)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling Human IgG with purified ab109489 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (AB109489)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (AB109489)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (AB109489)

Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling human IgG with ab109489 at a concentration of 0.01µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins. ab109489 anti-Human IgG antibody [EPR4421] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

• WB

Lab

Western blot - Anti-Human IgG antibody [EPR4421] (AB109489)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Human IgG antibody [EPR4421] (ab109489) at 1/20000 dilution

Lane 1:

Human fetal thymus tissue lysate at 20 µg

Lane 2:

Human fetal tonsil tissue lysate at 20 µg

Lane 3:

Human fetal spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

• WB

Unknown

Western blot - Anti-Human IgG antibody [EPR4421] (AB109489)

All lanes:

Western blot - Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution

Lane 1:

IgG2 recombinant protein (10 ng protein loaded)

Lane 2:

IgG1 recombinant protein (10 ng protein loaded)

Lane 3:

IgG3 recombinant protein (10 ng protein loaded)

Lane 4:

IgG4 recombinant protein (10 ng protein loaded)

Predicted band size: 36 kDa

false

• WB

Lab

Western blot - Anti-Human IgG antibody [EPR4421] (AB109489)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution

All lanes:

IgG3 recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

• WB

Lab

Western blot - Anti-Human IgG antibody [EPR4421] (AB109489)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Human IgG antibody [EPR4421] (ab109489) at 1/5000 dilution

Lane 1:

IgG1 recombinant protein at 0.01 µg

Lane 2:

IgG2 recombinant protein at 0.01 µg

Lane 3:

IgG4 recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false