抗人IgG抗体(ab2410)

We have not received any report of the this antibody being used for diagnosis of autoimmune diseases by IHC or other methods. It has been demonstrated to be capable of staining a sub-population of cells in tonsil sections, as shown on the datasheet, but detection of immune deposits of immunoglobulins may requirea more sensitive tecnique. If you do decide to try this antibody, please contact us if you have any difficulty.

Thank you for your call today and for your questions.

Here are the products that we discussed:

1) Rabbit anti-human IgG (H&L) antibody: Ab6715

https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Human-IgG-H-L-ab6715.html

2) Mouse anti-human IgG (H&L) antibody: Ab34834

https://www.abcam.com/Mouse-polyclonal-Secondary-Antibody-to-Human-IgG-H-L-ab34834.html

3) Anti-mouse IgG (H&L) antibody conjugated to AP: Ab7069

https://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7069.html

This anti-mouse IgG antibody is pre-adsorbed against human IgG, so that it will not bind to the human IgG on the Western blot. It will only bind to the mouse primary antibody.

I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you. Have a nice day.

Thank you for your reply.

I attached the MSDS to this email for your information.

I hope this ids helpful and wish you good luck with your research.

Thank you for your inquiry.

The MSDS PDFsfor our products can be found on the online datasheets.

If there is not MSDS as in the case of ab2410, this indicates that the product does not contains hazardous substances or only contains so little that a MSDS is not necessary.

If you still require a MSDS for ab2410, I will be happy to ask the department to generate one for you (which would say, that it is not hazardous in these minute amounts).

I hope this information is helpful and wish you a good day.

Vielen Dank für Ihre Nachrichten.

Leider sind die anti-human IgGs nicht die Kontrollen, die wir Ihnen als Isotypkontrolle für die IL9- und IL9R- Antikörper empfehlen würden. (Damit würden Sie sicher eine Färbung erhalten, die Sie so nicht beabsichtigen).

Als Isotypkontrolle können wir Ihnen ab27478 oder ab27472 (vorverdünnt=prediluted) empfehlen:

https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab27478.html (or use the following: https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab27478.html).

https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab27472.html (or use the following: https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab27472.html).

Es freut mich zu hören, dass Sie unser Angebot unsere Antikörper ab106287und ab111915an Gefrierschnitten zu testen, annehmen möchten.



Wie besprochen, können Sie den Gutscheincode zum Kauf jeweils eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in IHC-Fr eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: https://www.abcam.com/collaborationdiscount

Thank you for contacting us. I have heard back from the lab with the following information: This antibody was only tested on human tissues and not on any other species. Therefore, we do not know if it would crossreact with mouse or not. I am sorry to not have more helpful information for you in this regard. Please let me know if you would be looking into other proteins that might show low homology between human and mouse, and I can help you to find a suitable antibody. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us and I'm sorry to hear that you're having some trouble staining with ab2410. We haven't tested this antibody using monkey tissue, only human tissue (tonsil), so we haven't optimized the protocol for monkey. However, I've included some info about the protocol tested for you to try optimizing. Pretreatment enzymatic retrieval: We recommend histozyme treatment (Diagnostic Biosystem cat. #: K 046) for ab2410 for 5 minutes at room temperature. The antibody should be diluted even further if you're seeing background. Try 1/3000 - 1/5000 and dilute your secondary more too. The lab suggests incubating the primary for 30 mins at room temperature. Plus, what are you using for blocking? We'd recommend 5-10% serum from the species your secondary is raised in and incubating that for 1 hr at RT. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.