AB48536

Anti-Human Ig light chain 抗体 [MHK-49]

Name: 人Ig light chain抗体[MHK-49] (ab48536)| Abcam中文官网
Brand: Abcam Limited
SKU: AB48536
Price: 4514 CNY
Availability: InStock

Anti-Human Ig light chain antibody [MHK-49]

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(1 Publication)

Mouse Monoclonal IGKC antibody. Suitable for Flow Cyt, ELISA, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Human IGKC.

查看别名

Immunoglobulin kappa constant, Ig kappa chain C region, Ig kappa chain C region AG, Ig kappa chain C region CUM, Ig kappa chain C region EU, Ig kappa chain C region OU, Ig kappa chain C region ROY, Ig kappa chain C region TI, IGKC

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

MHK-49

亚型

IgG1

轻链类型

kappa

不含载体蛋白

反应种属

Human

应用

Flow Cyt, ELISA, ICC/IF

applications

免疫原

Full Length Protein corresponding to Human IGKC. The exact immunogen used to generate this antibody is proprietary information.

P01834

特异性

This antibody is specific for both soluble and membrane human immunoglobulin light chain kappa. It does not react with human immunoglobulin light chain lambda or heavy chain mu.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "This antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

存储溶液

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The human immunoglobulin light chain often referred to as "Ig light chain" or "IgL" is a component of antibodies. It's an essential part of the adaptive immune system. The light chains combine with heavy chains to form the complete immunoglobulin molecule. It has approximately 25 kDa mass. The light chain can be kappa (κ) or lambda (λ) types depending on the sequence. Expression occurs mainly in B lymphocytes where it plays a critical role in antibody diversity and specificity.

Biological function summary

The light chain contributes to antigen recognition by forming part of the antigen-binding site on an antibody. It's not a standalone entity but part of a larger immunoglobulin complex. This allows antibodies to bind specifically to antigens facilitating the immune response. The light chain also participates in stabilizing the antibody structure and ensuring proper immune function.

Pathways

The Ig light chain functions in the context of the adaptive immune system particularly in the immunoglobulin gene rearrangement and affinity maturation pathways. It works closely with the heavy chain to achieve effective antigen recognition. Additionally it interacts indirectly with proteins like B-cell receptor (BCR) which is important in the B lymphocyte signaling pathway. These interactions allow for the antibody-mediated detection and response to pathogens.

Abnormal Ig light chain production correlates with multiple myeloma and light chain amyloidosis. In multiple myeloma malignant plasma cells overproduce light chains often leading to their deposition in tissues and subsequent organ damage. In light chain amyloidosis the excess light chains misfold and deposit as amyloid fibrils causing organ dysfunction. Associations between these conditions and other immunoglobulin proteins highlight the role of Ig light chains in pathological processes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGKC

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and vaccine immunology : CVI 16:1327-31 PubMed19605595

2009

Development and validation of a fluorescent microsphere immunoassay for soluble CD30 testing.

Applications

Unspecified application

Species

Unspecified reactive species

Igor Pavlov,Thomas B Martins,Julio C Delgado

View all publications

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Anti-Human Ig light chain 抗体 [MHK-49]

Anti-Human Ig light chain antibody [MHK-49]

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

反应种属

应用

免疫原

特异性