AB184267

重组Anti-HuD + HuC抗体[EPR19098]

Name: HuD + HuC抗体[EPR19098] (ab184267)| Abcam中文官网
Brand: Abcam Limited
SKU: AB184267
Price: 2796 CNY
Availability: InStock
Rating: 5 (4 reviews)

Anti-HuD + HuC antibody [EPR19098]

RabMAb
Recombinant
了解详情

(4 Reviews)

(28 Publications)

Anti-HuD + HuC antibody [EPR19098] (ab184267) is a rabbit monoclonal antibody detecting HuC in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

查看别名

HUC, PLE21, ELAVL3, ELAV-like protein 3, Hu-antigen C, Paraneoplastic cerebellar degeneration-associated antigen, Paraneoplastic limbic encephalitis antigen 21, HuC

22 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nuclear and weakly cytoplasmic staining on myenteric ganglia of human colon is observed [PMID : 16918730].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-HuD + HuC antibody [EPR19098] (AB184267)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling HuC + HuD with ab184267 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic and nuclear staining on SH-SY5Y cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling HuC + HuDwith ab184267 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nuclear and weakly cytoplasmic staining on human glioma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nuclear and weakly cytoplasmic staining on neurons of human cerebral cortex is observed [PMID : 22007133].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling HuC + HuD with ab184267 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic and nuclear staining on Neuro-2a cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat colon tissue labeling HuC + HuD with ab184267 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Cytoplasmic and nuclear staining on myenteric ganglia of rat colon is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebral cortex tissue labeling HuC + HuD with ab184267 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Cytoplasmic and nuclear staining on neurons of mouse cerebral cortex is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebral cortex tissue labeling HuC + HuD with ab184267 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Cytoplasmic and nuclear staining on neurons of rat cerebral cortex is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Neuro-2a (Mouse neuroblastoma cell line) cells labeling HuC + HuDwith ab184267 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling HuC + HuD with ab184267 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Cytoplasmic and nuclear staining on myenteric ganglia of mouse stomach is observed (white arrow).

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nuclear and weakly cytoplasmic staining on neurons of rat cerebral cortex is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic and nuclear staining on myenteric ganglia of mouse stomach is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic and nuclear staining on myenteric ganglia of rat colon is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nuclear and weakly cytoplasmic staining on neurons of mouse cerebral cortex is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Indirect ELISA - Anti-HuD + HuC antibody [EPR19098] (AB184267)

I-ELISA

Lab

Indirect ELISA - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Indirect ELISA showing primary antibody ab184267 binding to Mouse Elavl1 (HuR); Mouse Elavl2 (HuB); Mouse Elavl3 (HuC); Mouse Elavl4 (HuD). Antigen concentration is 1000 ng/ml. Substrate solution is p-nitrophenyl phosphate(PNPP). Binding of ab184267 was assessed in a serial dilution range 1000-0 ng/ml. Binding was detected using the secondary antibody, Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.

Supplier Data

Immunoprecipitation - Anti-HuD + HuC antibody [EPR19098] (AB184267)

HuC + HuD was immunoprecipitated from 0.35 mg of mouse brain lysate with ab184267 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab184267 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse brain lysate, 10 μg (Input).

Lane 2 : ab184267 IP in mouse brain lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184267 in mouse brain lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-HuD + HuC antibody [EPR19098] (ab184267)

Observed band size: 39 kDa

false

Supplier Data

Western blot - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile is consistent with what has been described in the literature (PMID : 9016658; PMID : 8535975).

All lanes:

Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Observed band size: 39 kDa

false

Exposure time: 8s

Supplier Data

Western blot - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Blocking/Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267) at 1/5000 dilution

Lane 1:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 39 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1-3 : 1 minute; Lane 4-6 : 5 seconds.

The expression profile is consistent with what has been described in the literature (PMID : 9016658; PMID : 8535975).

All lanes:

Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat heart lysate at 10 µg

Lane 6:

Rat kidney lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 39 kDa

false

Lab

Western blot - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab184267 shows very weak cross reactivity with HuB. Band around 80kda detected by anti-His tag antibody should be dimer.

Lanes 1 - 9:

Western blot - Anti-HuD + HuC antibody [EPR19098] - BSA and Azide free (<a href='/products/primary-antibodies/hud-huc-antibody-epr19098-bsa-and-azide-free-ab232416'>ab232416</a>)

Lanes 1 - 9:

Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267) at 1/1000 dilution

Lane 1:

His-tagged Mouse HUR recombinant protein (aa1-326) at 10 ng

Lane 2:

His-tagged Mouse HUR recombinant protein (aa1-326) at 50 ng

Lane 3:

His-tagged Mouse HUR recombinant protein (aa1-326) at 100 ng

Lane 4:

His-tagged mouse HuB recombinant protein (aa1-360) at 10 ng

Lane 5:

His-tagged mouse HuB recombinant protein (aa1-360) at 50 ng

Lane 6:

His-tagged mouse HuB recombinant protein (aa1-360) at 100 ng

Lane 7:

Blank

Lane 8:

His-tagged mouse HuC recombinant protein (aa1-367) at 10 ng

Lane 9:

His-GST-tagged mouse HuD recombinant protein (aa1-385) at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 40 kDa

Observed band size: 37 kDa,75 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 0.5 second.

Please note that the band around 55kDa in lane 2 is most likely the HuD protein with tags.

All lanes:

Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267) at 1/2000 dilution

Lane 1:

His-tagged mouse HuC recombinant protein (aa1-367) at 0.01 µg

Lane 2:

His-GST-tagged mouse HuD recombinant protein (aa1-385) at 0.01 µg

Lane 3:

His-tagged mouse HuR recombinant protein (aa1-360) at 0.01 µg

Lane 4:

His-tagged mouse HuB recombinant protein (aa1-360) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 39 kDa,68 kDa

false

不同偶联物与剂型 (3)

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-HuD + HuC antibody [EPR19098]
Carrier free

Anti-HuD + HuC antibody [EPR19098] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-HuD + HuC antibody [EPR19098]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19098

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

Flow Cyt (Intra), ICC/IF, IHC-P, I-ELISA, WB, IHC-Fr, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody shows very weak cross reactivity to HuB in WB test. Please contact our Scientific support team for more information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1000 ng/mL", "IELISA-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

style

left-column

产品详情

What is this antibody validated in?
Anti-HuD + HuC antibody [EPR19098] (ab184267) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Mouse, Rat samples.

What is the molecular weight of HuC?
Anti-HuD + HuC [EPR19098] (ab184267) specifically detects a band for HuC (UniProt: Q60900) at a molecular weight of 40kDa.

Trusted by the scientific community
Anti-HuD + HuC [EPR19098] (ab184267) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The HuD and HuC proteins also known as neuronal ELAV-like RNA binding proteins play critical roles in neuronal development and function. These proteins weigh approximately 37 kDa and are specifically expressed in neurons. They are part of the family of ELAV (Embryonic Lethal Abnormal Vision) proteins with HuD sometimes referred to as C HuD. HuD and HuC are involved in RNA stabilization processes by binding to AU-rich elements in the 3' untranslated regions (3' UTR) of mRNA which influence the regulation of mRNA turnover stability and translation.

Biological function summary

HuD and HuC proteins are integral to the regulation of gene expression during neuronal differentiation. They participate in RNA processing machinery and can form complexes with other RNA-binding proteins affecting the neuronal mRNA transcriptome. These interactions facilitate the stabilization and localization of mRNAs essential for synaptic plasticity and neuron functionality. Their presence highlights the post-transcriptional regulation mechanisms vital for nervous system development and function.

Pathways

HuD and HuC proteins are essential players in neuron-specific mRNA processing pathways. They interact closely with molecular pathways involved in learning and memory particularly those involving the MAPK/ERK pathway. They frequently co-regulate mRNA with other proteins like HuR a ubiquitous ELAV protein. These interactions assist in the modulation of gene expression profiles necessary for proper neuronal signaling and plasticity.

HuD and HuC proteins are linked with conditions such as neurological cancer and neurodegenerative disorders. Their deregulation has been associated with neuroblastoma where aberrant expression influences tumor proliferation. Additionally alterations in HuD and HuC protein function have connections with Alzheimer's disease wherein their interactions with tau protein impact disease progression. Understanding these associations provides insights into potential therapeutic targets and interventions for such conditions.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

RNA-binding protein that binds to AU-rich element (ARE) sequences of target mRNAs, including VEGF mRNA (PubMed : 10710437). May also bind poly-A tracts via RRM 3 (By similarity). May be involved in neuronal differentiation and maintenance (By similarity). Plays a role in the stabilization of GAP43 mRNA and in spatial learning (By similarity).

See full target information ELAVL3

其他靶点

ELAVL4

style

left-column

文献 (28)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112885 PubMed40678517

2025

Gpr37 modulates the severity of inflammation-induced GI dysmotility by regulating enteric reactive gliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Keiramarie Robertson,Oliver Hahn,Adarsh Tantry,Beatriz G Robinson,Arwa T Faruk,Mathangi Janakiraman,Hong Namkoong,Kwangkon Kim,Jiayu Ye,Estelle Spear Bishop,Randy A Hall,Tony Wyss-Coray,Laren Becker,Julia A Kaltschmidt

Nature neuroscience 28:1146-1159 PubMed40442499

2025

The transcriptomes, connections and development of submucosal neuron classes in the mouse small intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Khomgrit Morarach,Ziwei Liu,Sanghita Banerjee,Yanan Chen,Ashley L Harb,Joel M Kosareff,Charles R Hall,Fernando López-Redondo,Elham Jalalvand,Suad H Mohamed,Anastassia Mikhailova,David R Linden,Ulrika Marklund

PeerJ 13:e19329 PubMed40292108

2025

Three distinct classes of myenteric ganglia in mice and humans: insights from quantitative analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Luyao Wu,Lei Xiang,Yingjian Chen,Handan Mao,Xinyao Meng,Jing Wang,Honglin Li,Xuyong Chen,Jiexiong Feng,Jun Xiao

Scientific reports 15:13612 PubMed40253554

2025

MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jiamin Zhao,Shuangyi Zhang,Yunjie Bai,Zhiguo Gong,Wenhui Bao,Zhuoya Yu,Bo Liu,Wei Mao,Surong Hasi

Cell & bioscience 15:43 PubMed40217547

2025

The influence of SARS-CoV-2 spike protein exposure on retinal development in the human retinal organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Gong,Lingling Ge,Yuxiao Zeng,Cao Yang,Yushan Luo,Jiahui Kang,Ting Zou,Haiwei Xu

Biomedicines 12: PubMed39457659

2024

Intestinal Region-Dependent Impact of NFκB-Nrf Crosstalk in Myenteric Neurons and Adjacent Muscle Cells in Type 1 Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Bence Pál Barta,Benita Onhausz,Abigél Egyed-Kolumbán,Afnan Al Doghmi,János Balázs,Zita Szalai,Ágnes Ferencz,Edit Hermesz,Mária Bagyánszki,Nikolett Bódi

Frontiers in pharmacology 15:1411642 PubMed39139632

2024

The 5HT4R agonist velusetrag efficacy on neuropathic chronic intestinal pseudo-obstruction in PrP-SCA7-92Q transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Liu,Yunfei Wu,Dewan Ren,Yulong Tao,Fangyi Mai,Jingyi Zhu,Xiang Li,Emanuela Colla,Maria Grimaldi,Roberto Giovannini,Fabrizio Giorgi,Loredana Vesci

Communications biology 6:1209 PubMed38012384

2023

Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondii.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Grochow,Britta Beck,Zaida Rentería-Solís,Gereon Schares,Pavlo Maksimov,Christina Strube,Lisa Raqué,Johannes Kacza,Arwid Daugschies,Simone A Fietz

NPJ Regenerative medicine 8:36 PubMed37443319

2023

MAP4Ks inhibition promotes retinal neuron regeneration from Müller glia in adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Houjian Zhang,Yuli Guo,Yaqiong Yang,Yuqian Wang,Youwen Zhang,Jingbin Zhuang,Yuting Zhang,Mei Shen,Jiankai Zhao,Rongrong Zhang,Yan Qiu,Shiying Li,Jiaoyue Hu,Wei Li,Jianfeng Wu,Haiwei Xu,Steven J Fliesler,Yi Liao,Zuguo Liu

British journal of pharmacology 180:2550-2576 PubMed37198101

2023

BQ788 reveals glial ET receptor modulation of neuronal cholinergic and nitrergic pathways to inhibit intestinal motility: Linked to postoperative ileus.

Applications

Unspecified application

Species

Unspecified reactive species

Elvio Mazzotta,Iveta Grants,Egina Villalobos-Hernandez,Samhita Chaudhuri,Jonathon L McClain,Luisa Seguella,Derek M Kendig,Bryan A Blakeney,Srinivasa K Murthy,Reiner Schneider,Patrick Leven,Sven Wehner,Alan Harzman,John R Grider,Brian D Gulbransen,Fedias L Christofi

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-HuD + HuC抗体[EPR19098]

Anti-HuD + HuC antibody [EPR19098]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunocytochemistry/ Immunofluorescence - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Flow Cytometry (Intracellular) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunocytochemistry/ Immunofluorescence - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Flow Cytometry (Intracellular) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Indirect ELISA - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Immunoprecipitation - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Western blot - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Western blot - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Western blot - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Western blot - Anti-HuD + HuC antibody [EPR19098] (AB184267)

Western blot - Anti-HuD + HuC antibody [EPR19098] (AB184267)

不同偶联物与剂型 (3)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

其他靶点

文献 (28)

搭配使用产品

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam Product Promise