重组Anti-HtrA2 / Omi抗体[E55] (ab32092)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E55] to HtrA2 / Omi
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-HtrA2 / Omi抗体[E55]
参阅全部 HtrA2 / Omi 一抗 -
描述
兔单克隆抗体[E55] to HtrA2 / Omi -
宿主
Rabbit -
特异性
ab32092 does not cross-react with other peptidase S1C family members. -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human HtrA2/ Omi aa 100-200 (N terminal). The exact sequence is proprietary.
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阳性对照
- Jurkat cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E55 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32092于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
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IHC-P |
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
IHC-P
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive. -
组织特异性
Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid. -
疾病相关
Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:610297]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. -
序列相似性
Belongs to the peptidase S1B family.
Contains 1 PDZ (DHR) domain. -
结构域
The mature N-terminus is involved in the interaction with XIAP.
The PDZ domain mediates interaction with MXI2. -
翻译后修饰
Autoproteolytically activated. -
细胞定位
Mitochondrion intermembrane space. Mitochondrion membrane. Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID. - Information by UniProt
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数据库链接
- Entrez Gene: 27429 Human
- Entrez Gene: 64704 Mouse
- Entrez Gene: 297376 Rat
- Omim: 606441 Human
- SwissProt: O43464 Human
- SwissProt: Q9JIY5 Mouse
- Unigene: 469045 Human
- Unigene: 21880 Mouse
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别名
- High temperature requirement protein A2 antibody
- HTRA 2 antibody
- HtrA like serine protease antibody
see all
图片
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Anti-HtrA2 / Omi antibody [E55] (ab32092) at 1/2000 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Predicted band size: 38 kDa
Observed band size: 38 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [E55] (ab32092)
ab32092 at a 1:250 dilution staining Htr2A/ Omi in human lung adenocarcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab32092 被引用在 17 文献中.
- Cho N et al. A novel splicing variant of DJ-1 in Parkinson's disease induces mitochondrial dysfunction. Heliyon 9:e14039 (2023). PubMed: 36915530
- Zhou W et al. HtrA2/Omi mitigates NAFLD in high-fat-fed mice by ameliorating mitochondrial dysfunction and restoring autophagic flux. Cell Death Discov 8:218 (2022). PubMed: 35449197
- Wang X Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide-induced acute pneumonia in rats. Mol Med Rep 22:3127-3134 (2020). PubMed: 32945404
- Ouyang H et al. Inhibitory effect of melatonin on Mst1 ameliorates myocarditis through attenuating ER stress and mitochondrial dysfunction. J Mol Histol 50:405-415 (2019). PubMed: 31256303
- He M et al. Lipopolysaccharide induces human olfactory ensheathing glial apoptosis by promoting mitochondrial dysfunction and activating the JNK-Bnip3-Bax pathway. Cell Stress Chaperones 24:91-104 (2019). PubMed: 30374881