重组Anti-htrA1抗体[EPR23240-64] (ab274322)
![Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)](https://www.abcam.cn/ps/products/274/ab274322/Images/ab274322-5-anti-htra1-antibody-epr23240-64-western-blot-wt-hap1.jpg "Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23240-64] to htrA1
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-htrA1抗体[EPR23240-64]
参阅全部 htrA1 一抗 -
描述
兔单克隆抗体[EPR23240-64] to htrA1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: SK-OV-3 whole cell lysate; Human brain, placenta, ovary and ovary cancer tissue lysates. IP: Human placenta tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23240-64 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab274322于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
|
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| IP |
1/30.
|
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
|
IP
1/30. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
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功能
Protease that regulate the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Represses signaling by TGF-beta family members. -
组织特异性
Expressed in a variety of tissues, with strongest expression in placenta. -
疾病相关
Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7) [MIM:610149]. ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians.
Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL) [MIM:600142]. CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, arteriosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries. -
序列相似性
Belongs to the peptidase S1B family.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
Contains 1 PDZ (DHR) domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 5654 Human
- Omim: 602194 Human
- SwissProt: Q92743 Human
- Unigene: 501280 Human
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别名
- ARMD7 antibody
- CARASIL antibody
- High-temperature requirement A serine peptidase 1 antibody
see all
图片
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Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)All lanes : Anti-htrA1 antibody [EPR23240-64] (ab274322) at 1/1000 dilution
Lane 1 : Wild-type HAP1 lysate
Lane 2 : HTRA1 Knockout HAP1 lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
ab274322 was shown to react with HTRA1 in wild-type HAP1 cells in Western blot with loss of signal observed in a HTRA1 knockout cell line. Wild-type HAP1 and HTRA1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab274322 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies. -
![Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)](https://www.abcam.cn/ps/products/274/ab274322/Images/ab274322-396357-anti-htra1-antibody-epr2324064-western-blot-human-brain-tissue-lysate-20-ug.jpg)
Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)All lanes : Anti-htrA1 antibody [EPR23240-64] (ab274322) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human placenta tissue lysate
Lane 3 : Human ovary tissue lysate
Lane 4 : Human ovary cancer tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 16767218, 19103920).
Exposure time: 48 seconds.
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![Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] (ab274322)](https://www.abcam.cn/ps/products/274/ab274322/Images/ab274322-1-anti-htra1-antibody-epr2324064-immunoprecipitation-human-placentalysate-10-ug.jpg)
Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] (ab274322)htrA1 was immunoprecipitated from 0.35 mg Human placenta lysate with ab274322 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab274322 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human placenta lysate 10 ug
Lane 2: ab274322 IP in Human placenta lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab274322 in Human placenta lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7.75 seconds.
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![Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)](https://www.abcam.cn/ps/products/274/ab274322/Images/ab274322-396356-anti-htra1-antibody-epr2324064-western-blot-skov3.jpg)
Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)All lanes : Anti-htrA1 antibody [EPR23240-64] (ab274322) at 1/1000 dilution
Lane 1 : SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: HepG2, MCF7 (PMID: 14716297, 22761798).
Exposure time: 3 minutes.
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![Anti-htrA1 antibody [EPR23240-64] (ab274322)](https://www.abcam.cn/ps/products/274/ab274322/Images/ab274322-4-benefits-of-recombinant-antibodies.png)
Anti-htrA1 antibody [EPR23240-64] (ab274322)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab274322 被引用在 3 文献中.
- Chang YJ et al. CRISPR editing demonstrates rs10490924 raised oxidative stress in iPSC-derived retinal cells from patients with ARMS2/HTRA1-related AMD. Proc Natl Acad Sci U S A 120:e2215005120 (2023). PubMed: 37126685
- Liu Z et al. Melatonin regulates trophoblast pyroptosis, invasion and migration in preeclampsia by inhibiting HtrA1 transcription through the microRNA-520c-3p/SETD7 axis. Am J Reprod Immunol 87:e13523 (2022). PubMed: 35137483
- Fan Z et al. Circ_0011460 upregulates HTRA1 expression by sponging miR-762 to suppress HTR8/SVneo cell growth, migration, and invasion. Am J Reprod Immunol 86:e13485 (2021). PubMed: 34270834