Anti-HSV1 ICP8 Major DNA binding蛋白抗体[11E2] (ab20194)
![Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)](https://www.abcam.cn/ps/products/20/ab20194/Images/ab20194-212421-anti-hsv1-icp8-major-dna-binding-protein-antibody-11e2-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)")
Key features and details
- Mouse monoclonal [11E2] to HSV1 ICP8 Major DNA binding protein
- Suitable for: IP, WB, ICC/IF
- Reacts with: Herpes simplex virus
- Isotype: IgG1
概述
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产品名称
Anti-HSV1 ICP8 Major DNA binding蛋白抗体[11E2]
参阅全部 HSV1 ICP8 Major DNA binding protein 一抗 -
描述
小鼠单克隆抗体[11E2] to HSV1 ICP8 Major DNA binding蛋白 -
宿主
Mouse -
经测试应用
适用于: IP, WB, ICC/IFmore details -
种属反应性
与反应: Herpes simplex virus -
免疫原
Full length native protein (purified from U-35-VERO cells) (HSV).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
11E2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab20194于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | ||
| WB | ||
| ICC/IF | (1) |
| 说明 |
|---|
应用说明
ICC/IF: Use at an assay dependent dilution (PMID 18434395).
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 129 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 129 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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相关性
Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell. ICP8 is the major DNA binding protein of herpes simplex virus type 1. -
数据库链接
- Entrez Gene: 2703458 Herpes simplex virus
- SwissProt: P04296 Herpes simplex virus
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别名
- DBP antibody
- Herpes simplex virus type 1 DBP antibody
- HSV1 DBP antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)This image is courtesy of an anonymous Abreview
ab20194 staining HSV1 ICP8 Major DNA binding protein in Human U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)](https://www.abcam.cn/ps/products/20/ab20194/Images/ab20194-29522-anti-hsv1-icp8-major-dna-binding-protein-antibody-11e2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)Image from Ohta A et al., Virol J. 2011 Jul 26;8:365. Fig 5.; doi:10.1186/1743-422X-8-365; 26 July 2011, Virology Journal 2011, 8:365Immunofluorescence analysis of cells infected with HSV1, staining HSV1 ICP8 Major DNA binding protein (purple) with ab20194, 7 (left) or 17 (right) hours after infection.
Cells were permeabilized in 0.1% Triton X-100 in PBS for 5 min at room temperature before blocking with blocking buffer (4% goat serum, 1% BSA in PBS-Tween [0.05%]) for 30 min at room temperature. Samples were incubated with primary antibody (1/1000) and a fluorescence conjugated anti-mouse IgG was used to detect staining. -
![Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)](https://www.abcam.cn/ps/products/20/ab20194/Images/ab20194-29521-anti-hsv1-icp8-major-dna-binding-protein-antibody-11e2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)Image from Alazard-Dany N et al., PLoS Pathog. 2009 Mar;5(3):e1000340. Epub 2009 Mar 13. Fig 5.; doi:10.1371/journal.ppat.1000340; March 13, 2009, PLoS Pathog 5(3): e1000340.Immunofluorescence analysis of HeLa cells transfected with pTF3 and pRF, staining HSV1 ICP8 Major DNA binding protein with ab20194.
Cells were fixed in paraformaldehyde for 10 min and then permeabilized with 0.5% Triton X-100 for 30 min. The cells were blocked with 4% BSA + 0.2% Tween for 30 min before incubation for 1 hour at RT with primary antibody (1/200 diluted in PBS-T). An AlexaFluor®488-conjugated donkey anti-mouse IgG (1/2000) was used as the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (44)
ab20194 被引用在 44 文献中.
- Duffy C et al. Gastric herpes simplex virus type 1 infection is associated with functional gastrointestinal disorders in the presence and absence of comorbid fibromyalgia: a pilot case-control study. Infection 50:1303-1311 (2022). PubMed: 35445970
- Wu Y et al. Nuclear soluble cGAS senses double-stranded DNA virus infection. Commun Biol 5:433 (2022). PubMed: 35538147
- Wang Z et al. Oleanolic Acid Derivative AXX-18 Exerts Antiviral Activity by Inhibiting the Expression of HSV-1 Viral Genes UL8 and UL52. Viruses 14:N/A (2022). PubMed: 35746758
- Osaka R et al. VP26, a herpes simplex virus type 1 capsid protein, increases DNA methylation in COASY promoter region. Brain Behav Immun Health 26:100545 (2022). PubMed: 36345321
- Murray-Nerger LA et al. Lamin B1 acetylation slows the G1 to S cell cycle transition through inhibition of DNA repair. Nucleic Acids Res 49:2044-2064 (2021). PubMed: 33533922