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Microbiology Organism Virus DNA Virus double stranded DNA Virus Herpes simplex

Anti-HSV1 + HSV2 ICP5 Major Capsid蛋白抗体[3B6] - BSA and Azide free (ab6508)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (43)

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Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] - BSA and Azide free (ab6508)

    Key features and details

    • Mouse monoclonal [3B6] to HSV1 + HSV2 ICP5 Major Capsid Protein - BSA and Azide free
    • Suitable for: IHC-P, WB, ELISA, ICC/IF
    • Reacts with: Herpes simplex virus, Human herpesvirus 2
    • Isotype: IgG2b

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    概述

    • 产品名称

      Anti-HSV1 + HSV2 ICP5 Major Capsid蛋白抗体[3B6] - BSA and Azide free
    • 描述

      小鼠单克隆抗体[3B6] to HSV1 + HSV2 ICP5 Major Capsid蛋白- BSA and Azide free
    • 宿主

      Mouse
    • 经测试应用

      适用于: IHC-P, WB, ELISA, ICC/IFmore details
    • 种属反应性

      与反应: Herpes simplex virus, Human herpesvirus 2
    • 免疫原

      Tissue, cells or virus corresponding to HSV1 + HSV2 ICP5 Major Capsid Protein.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.40
      Constituent: PBS
    • 无载体

      是
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 克隆

      单克隆
    • 克隆编号

      3B6
    • 骨髓瘤

      NS1/1-Ag4-1
    • 同种型

      IgG2b
    • 轻链类型

      kappa
    • 研究领域

      • Microbiology
      • Organism
      • Virus
      • DNA Virus
      • double stranded DNA Virus
      • Herpes simplex

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6508于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.

    Western Blot Titer: HSV-1 >1:3,200; HSV-2 >1:3,200.

    ELISA
    Use at an assay dependent concentration.

    ELISA titer: HSV-1 0.100 @ >1:102,400; HSV-2 0.100 @ >1:102,400.

    ICC/IF
    1/200. PubMed: 18701599

    IFA titer: HSV-1 1+ @ 1:3,200; HSV-2 1+ @ 1:3,200.

    说明
    IHC-P
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.

    Western Blot Titer: HSV-1 >1:3,200; HSV-2 >1:3,200.

    ELISA
    Use at an assay dependent concentration.

    ELISA titer: HSV-1 0.100 @ >1:102,400; HSV-2 0.100 @ >1:102,400.

    ICC/IF
    1/200. PubMed: 18701599

    IFA titer: HSV-1 1+ @ 1:3,200; HSV-2 1+ @ 1:3,200.

    靶标

    • 相关性

      Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) are two species of the herpes virus family, Herpesviridae, which cause infections in humans. They are also called Human Herpes Virus 1 and 2 (HHV-1 and HHV-2) and are neurotropic and neuroinvasive viruses; they enter and hide in the human nervous system, accounting for their durability in the human body.
    • 数据库链接

      • Entrez Gene: 1487302 Herpes simplex virus
      • Entrez Gene: 2703368 Herpes simplex virus
      • SwissProt: P06491 Herpes simplex virus
      • SwissProt: P89442 Herpes simplex virus
      • 别名

        • Capsid protein VP5 antibody
        • Herpes simplex virus 1 major capsid protein antibody
        • Herpes simplex virus 2 major capsid protein antibody
        • Human Herpes virus antibody
        • Major capsid protein antibody
        • MCP antibody
        • UL19 antibody
        • VP5 antibody
        see all

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] - BSA and Azide free (ab6508)
        Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] - BSA and Azide free (ab6508)Image from Ohta A et al., Virol J. 2011 Jul 26;8:365. doi: 10.1186/1743-422X-8-365.; Fig 4.; 26 July 2011, Virology Journal 2011, 8:365
        Immunofluorescence analysis of HSV infected cells, 7 hours (left) or 17 hours (right) post-infection, staining HSV1 + HSV2 ICP5 Major Capsid Protein with ab6508 at 1/200 dilution.

        Cells were fixed for 10 min in paraformaldehyde, permeabilized in 0.1% Triton X-100 and blocked in 4% goat serum, 1% bovine serum albumin, before incubating with primary antibody for 30 min at room temperature. An AlexaFluor®647-conjugated goat anti-mouse IgG1 was used as the secondary antibody.

      实验方案

      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (43)

      发表研究结果有使用 ab6508?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab6508 被引用在 43 文献中.

      • Duncan CJA  et al. Life-threatening viral disease in a novel form of autosomal recessive IFNAR2 deficiency in the Arctic. J Exp Med 219:N/A (2022). PubMed: 35442417
      • Chaturvedi S  et al. Disrupting autorepression circuitry generates "open-loop lethality" to yield escape-resistant antiviral agents. Cell 185:2086-2102.e22 (2022). PubMed: 35561685
      • Wang Y  et al. Single-cell RNA-sequencing analysis identifies host long noncoding RNA MAMDC2-AS1 as a co-factor for HSV-1 nuclear transport. Int J Biol Sci 16:1586-1603 (2020). PubMed: 32226304
      • Chen Y  et al. Single AAV-Mediated CRISPR-SaCas9 Inhibits HSV-1 Replication by Editing ICP4 in Trigeminal Ganglion Neurons. Mol Ther Methods Clin Dev 18:33-43 (2020). PubMed: 32577430
      • Wang W  et al. Guanidine modifications enhance the anti-herpes simplex virus activity of (E,E)-4,6-bis(styryl)-pyrimidine derivatives in vitro and in vivo. Br J Pharmacol 177:1568-1588 (2020). PubMed: 31709511
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Head and neck cancer, mouse xenograft)
      Specification
      Head and neck cancer, mouse xenograft
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate, pH=6
      Permeabilization
      No
      Blocking step
      Vector Lab Mouse Ig Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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      提交于 May 14 2013

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