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AB250818

重组Anti-HSPA4抗体[EPR14165] - BSA and Azide free

Anti-HSPA4 antibody [EPR14165] - BSA and Azide free

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Rabbit Recombinant Monoclonal HSPA4 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

查看别名

APG2, HSPH2, HSPA4, Heat shock 70 kDa protein 4, HSP70RY, Heat shock 70-related protein APG-2, Heat shock protein family H member 2

3 Images
Western blot - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (AB250818)
  • WB

Supplier Data

Western blot - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (AB250818)

This data was developed using ab185219, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HSPA4 antibody [EPR14165] (<a href='/products/primary-antibodies/hspa4-antibody-epr14165-ab185219'>ab185219</a>) at 1/5000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 94 kDa

false

Immunoprecipitation - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (AB250818)
  • IP

Supplier Data

Immunoprecipitation - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (AB250818)

This data was developed using ab185219, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from K562 cell lysate labeling HSPA4 using ab185219 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at a 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-HSPA4 antibody [EPR14165] (<a href='/products/primary-antibodies/hspa4-antibody-epr14165-ab185219'>ab185219</a>)

Predicted band size: 94 kDa

false

Flow Cytometry (Intracellular) - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (AB250818)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (AB250818)

This data was developed using ab185219, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed K562 cells labeling HSPA4 with ab185219 at 1/300 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

不同偶联物与剂型 (8)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14165

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, IP, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HSPA4 also known as Heat Shock Protein Family A Member 4 is a molecular chaperone involved in protein folding and stabilization during stress. This protein belongs to the heat shock protein family and has a molecular weight of approximately 70 kDa. HSPA4 is widely expressed across various tissues including the heart liver and brain. It operates within the cytoplasm and nucleus where it assists in protein homeostasis by preventing aggregation and misfolding of nascent polypeptides.
Biological function summary

HSPA4 plays a role in cellular stress responses and is part of the HSP70 chaperone complex. HSPA4 interacts with co-chaperones and other proteins to facilitate proper protein folding and degradation. It assists in the recovery of cells from thermal and oxidative stress. The protein ensures the stability of essential signal transduction proteins and supports protein quality control systems within the cell.

Pathways

HSPA4 integrates into the cellular stress response and protein degradation pathways. It engages with the ubiquitin-proteasome system aiding in the removal of damaged proteins. HSPA4 interacts with other heat shock proteins such as HSPA1A and HSPA5 coordinating stress response signals and maintaining cellular homeostasis. This involvement in protein maintenance links it to regulatory pathways that address protein misfolding and cellular repair processes.

HSPA4 shows connections to neurodegenerative diseases and cancer. Its role in protein homeostasis suggests involvement in Alzheimer's disease as protein misfolding stands as an important pathological feature. In cancer HSPA4 is associated with cell proliferation and survival mechanisms. It interacts with oncogene products potentially contributing to tumor progression. Studies explore HSPA4 as a therapeutic target assessing its potential influence in disease modulation through its interactions with other stress proteins and pathways.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information HSPA4
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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